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関連する概念動画

Mismatch Repair01:36

Mismatch Repair

Overview
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...
Genome Copying Errors02:46

Genome Copying Errors

DNA replication is a well-evolved process that copies millions of base pairs with high fidelity during each cell division. Occasionally a wrong base or a long stretch of wrong bases may get added to the daughter strands. If the errors are left unchecked, cells might accumulate several mutations that might endanger their  survival. Therefore, the copying errors are checked and repaired at three levels.
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...

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関連する実験動画

Updated: Jul 11, 2026

Visualization of UV-induced Replication Intermediates in E. coli using Two-dimensional Agarose-gel Analysis
10:36

Visualization of UV-induced Replication Intermediates in E. coli using Two-dimensional Agarose-gel Analysis

Published on: December 21, 2010

DNA損傷による複製フォークの回帰とEscherichia coliにおける処理

Justin Courcelle1, Janet R Donaldson, Kin-Hoe Chow

  • 1Department of Biological Sciences, Box GY, Mississippi State University, Mississippi State, MS 39762, USA. jcourcelle@biology.msstate.edu

Science (New York, N.Y.)
|January 25, 2003
PubMed
まとめ

DNAの損傷は複製フォークを停止し,修復を可能にするために逆転することができます. このフォーク回帰は,E. coliのRecAとRecFタンパク質によって安定させられ,DNA修復と細胞生存に不可欠である.

科学分野:

  • 分子生物学は分子生物学である.
  • 遺伝学 遺伝学とは
  • バイオケミストリー バイオケミストリー

背景:

  • 複製を阻害するDNA病変は,遺伝的不安定性と細胞死の主な原因です.
  • E. coli の紫外線損傷後の複製回復は,ReCAとrecF経路タンパク質に依存しています.

研究 の 目的:

  • DNAの病変に遭遇した後の複製フォークの回復のメカニズムを調査する.
  • この過程における RecA,RecF,RecQ,RecJタンパク質の役割を明らかにする.

主な方法:

  • 複製フォークのダイナミクスをin vivoで分析するために二次元アガロースゲル電泳を用いた.
  • フォークの安定性に対するrecA,recF,recQ,recJ遺伝子の変異の影響を調査した.

主要な成果:

  • 複製を阻害するDNAの病変は,複製フォークの一時的な逆転 (回帰) を誘発する.
  • 反転フォークの中間体は,ReCAとReCFによって安定化されます.
  • RecQ-RecJヘリカーゼ-ヌクレアゼの欠如は,反転フォークの中間物質の劣化につながります.

結論:

さらに関連する動画

Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter
06:59

Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter

Published on: March 31, 2022

Quantifying Replication Stress in Ovarian Cancer Cells Using Single-Stranded DNA Immunofluorescence
06:25

Quantifying Replication Stress in Ovarian Cancer Cells Using Single-Stranded DNA Immunofluorescence

Published on: February 10, 2023

関連する実験動画

Last Updated: Jul 11, 2026

Visualization of UV-induced Replication Intermediates in E. coli using Two-dimensional Agarose-gel Analysis
10:36

Visualization of UV-induced Replication Intermediates in E. coli using Two-dimensional Agarose-gel Analysis

Published on: December 21, 2010

Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter
06:59

Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter

Published on: March 31, 2022

Quantifying Replication Stress in Ovarian Cancer Cells Using Single-Stranded DNA Immunofluorescence
06:25

Quantifying Replication Stress in Ovarian Cancer Cells Using Single-Stranded DNA Immunofluorescence

Published on: February 10, 2023

  • 複製フォーク回帰は,DNA合成を阻害するDNA病変に対処するための重要なメカニズムです.
  • RecAやRecFのようなタンパク質によって媒介されるこのプロセスは,DNA修復酵素へのアクセスを可能にします.
  • 修復が成功すると,プロセシブ複製が再開され,細胞の生存能力が確保されます.