Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Recombinant DNA01:09

Recombinant DNA

Overview
Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
Heterochromatin02:38

Heterochromatin

The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at 9th...
Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Phosphorothioate-Free and Self-Interaction-Reduced Acyclic Nucleic Acids for Effective Antisense Oligonucleotide.

Journal of medicinal chemistry·2026
Same author

An acyclic nucleic acid-modified siRNA targeting CAG expansions for polyglutamine disease treatment.

Molecular therapy. Nucleic acids·2026
Same author

Membrane-Penetrating Molecular Device Based on a Triplex Containing Acyclic L-Threoninol Nucleic Acid Functionalized with Cholesterol.

Angewandte Chemie (International ed. in English)·2025
Same author

Staple oligomers induce a stable RNA G-quadruplex structure for protein translation inhibition in therapeutics.

Nature biomedical engineering·2025
Same author

Reversible photocycloaddition of 8-pyrenylvinylguanine for photoreactive serinol nucleic acid (SNA).

Organic & biomolecular chemistry·2025
Same author

Highly Effective Chemical Ligation of DNA and l-aTNA.

Current protocols·2025

関連する実験動画

Updated: Jun 25, 2026

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time
14:36

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time

Published on: August 26, 2009

制御可能なH結合のためのDNA染色コンジュガット (((1))

Hiroyuki Asanuma1, Kenji Shirasuka, Tohru Takarada

  • 1Research Center for Advanced Science and Technology, The University of Tokyo, Komaba, Meguro-ku, Tokyo 153-8904, Japan. asanuma@mkomi.rcast.u-tokyo.ac.jp

Journal of the American Chemical Society
|February 20, 2003
PubMed
まとめ

研究者はDNA-メチルレッド結合体を合成し,H集積を形成しました. これらのDNA染料集積物のスペクトル特性は,DNA二重複形成によって逆転的に変化し,集積された構造に対する制御を示しています.

さらに関連する動画

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
07:44

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes

Published on: July 6, 2016

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

関連する実験動画

Last Updated: Jun 25, 2026

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time
14:36

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time

Published on: August 26, 2009

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
07:44

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes

Published on: July 6, 2016

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

科学分野:

  • 超分子化学 超分子化学
  • バイオ物理化学 バイオ物理化学
  • マテリアルサイエンス 材料科学

背景:

  • DNAベースのナノマテリアルは,ユニークな構造特性を提供します.
  • DNAスキャフォールド内の染料の集積を制御することは,機能的なアプリケーションにとって極めて重要です.
  • メチル赤色染料は,異なるスペクトルシグネチャーを持つHアグレガートを形成することができます.

研究 の 目的:

  • DNA結合体内のメチルレッドH集合体を合成し,特徴づけること.
  • H集積形成とスペクトル特性に対するDNA構造の影響を調査する.
  • DNAハイブリッド化によるH集積構造の可逆制御を実証する.

主な方法:

  • 多数のメチル赤分子のDNA結合体の合成.
  • UV-Visスペクトロスコーピーは,スペクトルの変化をモニタリングします.
  • DNA二重構造の形成を評価するための熱性デナチュレーション研究 (T(m))

主要な成果:

  • DNA-メチルレッドコンジュガートを合成し,Hアグレガートを形成しました.
  • 染料の組み込みが増加するにつれて,重要なヒプソクロミシティと帯域の縮小が観察され,H集積物の形成を示しています.
  • DNA複合体の形成と解離時に,反転可能なスペクトル変化 (H帯の消失と再出現) が実証されている.

結論:

  • DNA-メチルレッド結合体は,明確に定義されたH集合体を形成することができます.
  • これらの集積物のスペクトル特性は,DNAの構造変化に敏感である.
  • DNAハイブリデーションは,メチルレッドHの集積形成と構造を制御するための可逆的なメカニズムを提供します.