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関連する概念動画

Ligand Binding and Linkage00:49

Ligand Binding and Linkage

Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked.  In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence the...
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Ligand Binding and Linkage00:49

Ligand Binding and Linkage

Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked.  In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence the...
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...

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関連する実験動画

Updated: Jul 6, 2026

Monitoring the Assembly of a Secreted Bacterial Virulence Factor Using Site-specific Crosslinking
11:33

Monitoring the Assembly of a Secreted Bacterial Virulence Factor Using Site-specific Crosslinking

Published on: December 17, 2013

タンパク質の相互作用を探求するためのモジュラークロスリンクアプローチ.

Michelle Trester-Zedlitz1, Katsuhiko Kamada, Stephen K Burley

  • 1Laboratories of Synthetic Protein Chemistry, Rockefeller University, 1230 York Avenue, New York, NY 10021, USA.

Journal of the American Chemical Society
|February 27, 2003
PubMed
まとめ
この要約は機械生成です。

この研究は,高度なクロスリンク反応剤と質量スペクトロメトリを使用して,タンパク質とタンパク質の相互作用をマッピングするための新しい方法を導入しています. この技術は,負のコファクター2複合体内の相互作用をうまく解明しました.

さらに関連する動画

A Modified Yeast-one Hybrid System for Heteromeric Protein Complex-DNA Interaction Studies
10:47

A Modified Yeast-one Hybrid System for Heteromeric Protein Complex-DNA Interaction Studies

Published on: July 24, 2017

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies
10:01

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies

Published on: November 28, 2017

関連する実験動画

Last Updated: Jul 6, 2026

Monitoring the Assembly of a Secreted Bacterial Virulence Factor Using Site-specific Crosslinking
11:33

Monitoring the Assembly of a Secreted Bacterial Virulence Factor Using Site-specific Crosslinking

Published on: December 17, 2013

A Modified Yeast-one Hybrid System for Heteromeric Protein Complex-DNA Interaction Studies
10:47

A Modified Yeast-one Hybrid System for Heteromeric Protein Complex-DNA Interaction Studies

Published on: July 24, 2017

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies
10:01

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies

Published on: November 28, 2017

科学分野:

  • バイオケミストリー バイオケミストリー
  • プロテオミクス プロテオミクスは,プロテオミクスの
  • 化学生物学 化学生物学とは

背景:

  • タンパク質とタンパク質の相互作用を理解することは,細胞メカニズムを解読する上で極めて重要です.
  • これらの相互作用を研究するための既存の方法は,範囲や感度が限られている可能性があります.

研究 の 目的:

  • タンパク質とタンパク質の相互作用の解明のための新しい統合的アプローチを開発し,検証する.
  • この方法を適用して,負のコファクター2複合体の構造的組織を調査する.

主な方法:

  • 新規のクロスリンク反応剤のためのモジュラー型固体相合成戦略.
  • マイクロコンセントレーターを用いてクロスリンクされたタンパク質の濃縮と消化.
  • 交絡ペプチドの質量スペクトロメトリック分析と計算分析を組み合わせた.

主要な成果:

  • 統合方法により,交互にリンクされたペプチドの識別が可能になった.
  • このアプローチは,ヘテロジメの負のコファクター2複合体内のクロスリンクを研究するために成功裏に適用されました.
  • これは,タンパク質の相互作用ネットワークをマッピングするための堅牢なプラットフォームを提供します.

結論:

  • 記述された方法は,タンパク質とタンパク質の相互作用を研究するための強力なツールを提供します.
  • このアプローチは,詳細な相互作用マッピングを通じて,複雑な生物学的システムの理解を高めます.
  • この技術は,様々なタンパク質複合体と生物学的文脈に広く適用できます.