Cdc42はGSK-3βおよびアデノマトス型ポリポシス・コリーを調節し,細胞の極性を制御する
Sandrine Etienne-Manneville1, Alan Hall
1MRC Laboratory for Molecular Cell Biology and Cell Biology Unit, Cancer Research UK Oncogene and Signal Transduction Group, and Department of Biochemistry and Molecular Biology, University College London, Gower Street, London WC1E 6BT, UK.
Nature
|March 1, 2003
まとめ
小型GTPase Cdc42は,グリコゲン合成キナーゼ-3β (GSK-3β) とアデノマトス型ポリポシス・コーライ (Apc) の細胞内の局所化を制御することによって,細胞の極性を調節する.
科学分野:
- 細胞生物学 細胞生物学
- 分子生物学は分子生物学である.
- バイオケミストリー バイオケミストリー
背景:
- 細胞の極性は,モルフォゲネシスと移住を含む真核細胞の機能にとって極めて重要です.
- Cdc42-Par6-aPKC複合体は細胞非対称性を確立することが知られているが,下流効果者は不明である.
研究 の 目的:
- 細胞移動におけるPar6-aPKC複合体の下流ターゲットを調査する.
- Cdc42が細胞の極性を調節する分子機構を解明する.
主な方法:
- 主要ラットアストロサイトは,細胞移動アッセイに使用されました.
- Par6-PKCzeta,GSK-3beta,およびApcの相互作用が分析されました.
- 微小管のダイナミクスと,先端のタンパク質の局所化は調査されました.
主要な成果:
- Par6-PKCzetaはGSK-3beta.と直接相互作用し,GSK-3beta.を調節する.
- Cdc42依存のGSK-3βフォスフォリレーションが先端で発生すると,センターソームの極化が促進されます.
- GSK-3βは,Apcと微小管プラス端との相互作用を調節し,細胞の極化に不可欠です.
結論:
- Cdc42は,GSK-3βとApcの空間的調節により,細胞の極性を制御する.
- マイクロチューブルの連結におけるAPCの役割は,細胞の二極化に極めて重要です.
- この経路は,Apcの腫瘍抑制機能に寄与する可能性がある.
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