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関連する概念動画

From DNA to Protein03:06

From DNA to Protein

The flow of genetic information in cells from DNA to mRNA to protein is described by the central dogma, which states that genes specify the sequence of mRNAs, which in turn specify the sequence of amino acids making up all proteins. The decoding of one molecule to another is performed by specific proteins and RNAs. Because the information stored in DNA is so central to cellular function, it makes intuitive sense that the cell would make mRNA copies of this information for protein synthesis...
Southern Blot02:57

Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...
DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

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Analyzing Large Protein Complexes by Structural Mass Spectrometry
15:35

Analyzing Large Protein Complexes by Structural Mass Spectrometry

Published on: June 20, 2010

プロテオミクスケールでのタンパク質分析

Eric Phizicky1, Philippe I H Bastiaens, Heng Zhu

  • 1University of Rochester School of Medicine, Department of Biochemistry and Biophysics, Box 712, 601 Elmwood Avenue, Rochester, New York 14642, USA. eric_phizicky@urmc.rochester.edu

Nature
|March 14, 2003
PubMed
まとめ
この要約は機械生成です。

タンパク質 (プロテオーム) の完全なセットを理解することは大きな課題です. タンパク質クローンを系統的に生成し分析することは,タンパク質の機能と相互作用を細胞全体の文脈で定義するために非常に重要です.

さらに関連する動画

Quantitative Analysis of Chromatin Proteomes in Disease
08:11

Quantitative Analysis of Chromatin Proteomes in Disease

Published on: December 28, 2012

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
08:53

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

関連する実験動画

Last Updated: Jul 3, 2026

Analyzing Large Protein Complexes by Structural Mass Spectrometry
15:35

Analyzing Large Protein Complexes by Structural Mass Spectrometry

Published on: June 20, 2010

Quantitative Analysis of Chromatin Proteomes in Disease
08:11

Quantitative Analysis of Chromatin Proteomes in Disease

Published on: December 28, 2012

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
08:53

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

科学分野:

  • プロテオミクス プロテオミクスは,プロテオミクスの
  • 分子生物学は分子生物学である.
  • システム生物学 システム生物学

背景:

  • タンパク質のアイデンティティ,数量,構造,機能を含むプロテオームの定義は,長期的な大きな課題です.
  • タンパク質の性質が異なる細胞環境でどのように変化するかを理解することは,生物学的洞察にとって不可欠です.

研究 の 目的:

  • タンパク質の包括的な定義を達成するための重要なステップを概説する.
  • タンパク質発現セットの系統的,全ゲノム分析の有用性を強調する.

主な方法:

  • プロテオームの代表的なタンパク質を発現するクローンセットの生成.
  • これらのタンパク質発現セットの全ゲノム分析.
  • 遺伝子,生化学,細胞生物学技術の応用.

主要な成果:

  • 様々な生物技術の体系的な応用を可能にします.
  • タンパク質に生化学的活動の割り当てを容易にする.
  • タンパク質配列の構築とタンパク質の相互作用の識別をサポートします.
  • 細胞のコンパートメント内のタンパク質の局所化を助けます.

結論:

  • タンパク質発現クローンの体系的な生成と分析は,プロテオミクスの進歩にとって不可欠です.
  • このアプローチは,プロテオームの包括的な機能的および相互作用的特徴付けのためのプラットフォームを提供します.
  • これは,細胞タンパク質の行動と組織をより深く理解するための道を開く.