バンコマイシン耐性エンテロコッカス・フェカリスの進化における移動DNAの役割
I T Paulsen1, L Banerjei, G S A Myers
1Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA. ipaulsen@tigr.org
Science (New York, N.Y.)
|March 29, 2003
まとめ
ヴァンコマイシン耐性菌Enterococcus faecalis V583のゲノムには,新しいヴァンB耐性トランポゾンとユニークなプラズミッドを含む,重要なモバイルDNAが含まれています. この遺伝的移動性は,エンテロコックスの抗生物質耐性が急速に広がるのを助けます.
科学分野:
- 微生物学 微生物学とは
- ゲノミクスゲノミクスとは
- 分子生物学は分子生物学である.
背景:
- Enterococcus faecalisは,病院で発症する感染症の一般的な原因です.
- 腸球菌におけるバンコミシン耐性症は,公衆衛生上の重大な懸念事項である.
- 抗生物質耐性の遺伝的基礎を理解することは,新しい治療法の開発に不可欠です.
研究 の 目的:
- バンコマイシン耐性臨床単離体であるEnterococcus faecalis V583の完全なゲノム配列を解析するために.
- 移動性遺伝子要素とその抗生物質耐性における潜在的な役割を特定する.
- V583ゲノム内の新しい耐性メカニズムとプラズミドを特徴付ける.
主な方法:
- Enterococcus faecalis V583.3.の全ゲノムシーケンシングについて
- トランポゾンやプラズミッドを含む移動性DNA要素を特定するためのバイオ情報分析.
- 新しい遺伝子や要素を特定するための比較ゲノミクス.
主要な成果:
- E. faecalis V583のゲノムの25%以上は,移動性または外来DNAで構成されています.
- 新しいバンBバンコマイシン耐性結合トランポゾンが特定されました.
- 3つのプラズミドが発見され,そのうち2つのフェロモン感知結合プラズミドが発見され,1つは新しいフェロモン阻害剤を有する.
結論:
- E. faecalis V583のゲノムは,移動性遺伝要素が豊富で,バンコマイシン耐性に貢献しています.
- バンBトランポゾンやユニークなプラズミドを含む新しい移動要素が特定されました.
- 移動性DNAの高い含有量は,エンテロコックスの抗生物質耐性の急速な獲得と拡散を容易にする可能性が高い.
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