ヒトの骨髄由来細胞をブカル上皮細胞に微分化 in vivo: 分子分析研究
Simon D Tran1, Stanley R Pillemer, Amalia Dutra
1National Institute of Dental and Craniofacial Research, Gene Therapy and Therapeutics Branch National Institutes of Health, Bethesda, MD 20892-1190, USA. stran@dir.nidcr.nih.gov <stran@dir.nidcr.nih.gov>
Lancet (London, England)
|April 4, 2003
まとめ
成人骨髄由来細胞は,受容細胞と融合することなく,体内では上皮細胞に変容することができます. これは,細胞の有意な可塑性を示し,再生医療の応用の可能性を示しています.
科学分野:
- 幹細胞生物学 幹細胞生物学とは
- 再生医学は再生医学である.
- 細胞の可塑性について
背景:
- 大人の骨髄由来細胞 (BMD) は,組織修復の可能性を示しています.
- 実験室内研究では,BMD細胞の可塑性に関する疑問が提起され,細胞の分化ではなく融合が示唆された.
- BMD細胞の分化における細胞融合のインビボの役割は不明のままでした.
研究 の 目的:
- 骨髄由来細胞が上皮組織を植民地化し,融合なしに分化できるかどうかを調査する.
- 成人幹細胞の可塑性における融合イベントのインビボの重要性を決定する.
主な方法:
- Y染色体とX染色体のプローブ ( 35-硫黄,ディゴキシジニン,または光でラベル付け) を使ってin-situハイブリダイゼーションを使用しました.
- 抗サイトケラチン13を用いた免疫ヒストケミストリーが実施された.
- 男性の骨髄または外周血液幹細胞移植を受けた女性からの口腔上皮細胞を分析した.
主要な成果:
- Y染色体陽性ブカル細胞は,移植後4~6年 (0.8-12.7%) のすべての女性受容者に検出されました.
- 非常に少数の細胞 (0.01%) が潜在的融合イベント (XY+XX細胞) を示した.
- 男性のBMD細胞は,女性受容者の口腔上皮細胞に成功して移行し,分化しました.
結論:
- 男性の骨髄由来細胞は頬に移動し,受容細胞と融合することなく上皮細胞に微分化します.
- このプロセスは,血液形成細胞またはストロマルの原始細胞の変異を表す可能性があります.
- BMD細胞の可塑性は,再生医療療法にとって有望である.
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