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Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
Anaphase Promoting Complex00:50

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.
Anaphase Promoting Complex00:50

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...

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ポリコンブタンパク質 Pc2 は SUMO E3 である.

Michael H Kagey1, Tiffany A Melhuish, David Wotton

  • 1Department of Biochemistry and Molecular Genetics, Center for Cell Signaling, University of Virginia, Charlottesville, VA 22908, USA.

Cell
|April 8, 2003
PubMed
まとめ
この要約は機械生成です。

ポリコンブタンパク質Pc2は,トランスクリプションのコアプレッサーであるCtBPのスモイル化を強化します. これは,Pc2がSUMO E3リガゼとして作用し,PcG体を重要なスモイレーションセンターとして位置づけることを示唆しています.

さらに関連する動画

In Vivo Detection and Analysis of Rb Protein SUMOylation in Human Cells
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In Vivo Detection and Analysis of Rb Protein SUMOylation in Human Cells

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In Vitro SUMOylation Assay to Study SUMO E3 Ligase Activity
09:45

In Vitro SUMOylation Assay to Study SUMO E3 Ligase Activity

Published on: January 29, 2018

関連する実験動画

Last Updated: May 7, 2026

Toxicological Assays for Testing Effects of an Epigenetic Drug on Development, Fecundity and Survivorship of Malaria Mosquitoes
10:26

Toxicological Assays for Testing Effects of an Epigenetic Drug on Development, Fecundity and Survivorship of Malaria Mosquitoes

Published on: January 16, 2015

In Vivo Detection and Analysis of Rb Protein SUMOylation in Human Cells
09:40

In Vivo Detection and Analysis of Rb Protein SUMOylation in Human Cells

Published on: November 2, 2017

In Vitro SUMOylation Assay to Study SUMO E3 Ligase Activity
09:45

In Vitro SUMOylation Assay to Study SUMO E3 Ligase Activity

Published on: January 29, 2018

科学分野:

  • 分子生物学は分子生物学である.
  • エピジェネティクス エピジェネティクス
  • タンパク質生化学 タンパク質生化学

背景:

  • ポリコンブ群 (PcG) のタンパク質は,PcG体と呼ばれる大きな複合体を通して遺伝子発現を調節する.
  • 人間のPcGタンパク質であるPc2は,PcG体内の転写性コアプレッサーCtBPと相互作用する.

研究 の 目的:

  • CtBP.の改変におけるPc2の役割を調査する.
  • Pc2がCtBPスモイレーションに影響するかどうかを判断し,そのメカニズムを解明する.

主な方法:

  • 精製されたタンパク質を用いたインビトロスモイレーション試験.
  • タンパク質の局所化とPcG体内の相互作用を調査するインビボ研究.
  • CtBP変異の生化学分析.

主要な成果:

  • CtBPは,単一のリジン残基でスモイル化されます.
  • CtBPのスモイル化には,SUMO E1,E2 (Ubc9) とSUMO-1が必要です.
  • Pc2はCtBPのスモイル化をインビトロおよびインビボで著しく強化します.
  • Pc2は,CtBPとUbc9をPcGの組織に勧誘することで,スモイル化を促進する.

結論:

  • Pc2はCtBPのためのSUMO E3リガゼとして機能する.
  • PcG体は,スモイレーション反応の中心として機能する.
  • このメカニズムは,遺伝子発現におけるPcGタンパク質の新たな規制的役割を強調しています.