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ユーカリオットの遺伝子発現におけるノイズ
William J Blake1, Mads KAErn, Charles R Cantor
1Center for BioDynamics, Center for Advanced Biotechnology, Bioinformatics Program, and Department of Biomedical Engineering, Boston University, 44 Cummington Street, Boston, Massachusetts 02215, USA.
Nature
|April 11, 2003
まとめ
ユカリオット遺伝子の発現は騒々しく,転写が細胞間多様性を引き起こす. この転写騒音は,プロカリオットとは異なり,翻訳レベルで制御され,細胞の分化に影響を与えます.
科学分野:
- 分子生物学は分子生物学である.
- システム生物学 システム生物学
- 遺伝学 遺伝学とは
背景:
- ユカリオットの遺伝子発現は定量的で確率的であり,細胞の異質性につながる.
- 遺伝子発現におけるストキャスティシティは,刺激に対する個々の細胞の反応の重要な要因である.
- 騒音源を理解することは,細胞の変動性を説明する上で極めて重要です.
研究 の 目的:
- 細胞細胞の異質性に対する転写的ストキャスティシティの貢献を研究する.
- 転写ノイズが翻訳レベルで調節できるかどうかを判断する.
- 遺伝子調節ネットワークと細胞の分化における騒音の役割を調査する.
主な方法:
- ユカリオットの転写開始のストキャスティックモデルを使用した.
- 分析されたパルサチルメッセンジャーRNA (mRNA) の生成と再起動.
- 遺伝子カスケードネットワークにおけるノイズ伝播を研究した.
- 実験的にSaccharomyces cerevisiaeで検証を行った.
主要な成果:
- 転写性ストキャスティシティは,プロカリオットと異なる真核細胞細胞の異質性に大きく貢献します.
- 再始動による脈動性mRNA生成は,トランスクリプション効率に対するノイズ依存性にとって重要である.
- 騒音は,翻訳レベルで調節することができます.
- 調節性タンパク質のトランスクリプションノイズの増加は,細胞間の変異性を増幅し,バイスタブルな発現状態を延長します.
結論:
- 転写ノイズは,真核細胞のクローン集団における異質性の主要な原動力である.
- ユーカリオットのノイズメカニズム,特に脈動性トランスクリプションは,プロカリオットと根本的に異なる.
- 翻訳レベルでの騒音調節は制御メカニズムを提供する.
- トランスクリプションノイズは,表型変異と細胞分化プロセスにおいて重要な役割を果たします.
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