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関連する概念動画

Histone Modification02:32

Histone Modification

The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
Spreading of Chromatin Modifications02:25

Spreading of Chromatin Modifications

The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer is an enzyme that can...
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
Heterochromatin02:38

Heterochromatin

The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at 9th...
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
Histone Modification02:32

Histone Modification

The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...

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関連する実験動画

Updated: Jun 23, 2026

Reconstitution of Nucleosomes with Differentially Isotope-labeled Sister Histones
09:26

Reconstitution of Nucleosomes with Differentially Isotope-labeled Sister Histones

Published on: March 26, 2017

CtBP共抑制体複合体によって媒介される協調ヒストンの改変.

Yujiang Shi1, Jun-ichi Sawada, Guangchao Sui

  • 1Department of Pathology, Harvard Medical School, 200 Longwood Avenue, Boston, Massachusetts 02115, USA. yang_shi@hms.harvard.edu

Nature
|April 18, 2003
PubMed
まとめ
この要約は機械生成です。

C末端結合タンパク質 (CtBP) 複合体は,DNAターゲティングとヒストン改変を通じて遺伝子抑制を媒介する. このメカニズムは,腫瘍形成と動物の発達における役割において極めて重要です.

さらに関連する動画

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
10:28

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries
10:10

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Published on: March 31, 2019

関連する実験動画

Last Updated: Jun 23, 2026

Reconstitution of Nucleosomes with Differentially Isotope-labeled Sister Histones
09:26

Reconstitution of Nucleosomes with Differentially Isotope-labeled Sister Histones

Published on: March 26, 2017

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
10:28

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries
10:10

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Published on: March 31, 2019

科学分野:

  • 分子生物学は分子生物学である.
  • エピジェネティクス エピジェネティクス
  • 癌生物学 癌生物学について

背景:

  • 転写共抑制体C端結合タンパク質 (CtBP) は腫瘍発生と動物の発達に関与しています.
  • CtBPは,PXDLSモチーフを持つ転写因子を介してDNAに採用されますが,その正確な機能は不明です.

研究 の 目的:

  • CtBP媒介による遺伝子抑制の分子メカニズムを特定する.
  • 腫瘍発生におけるCtBPの役割を調査する.

主な方法:

  • 遺伝子ターゲティングとヒストン改変能力を持つCtBP複合体の特定.
  • RNA媒介による干渉により,CtBP発現および関連する活動を阻害する.
  • E-カデリンのプロモーターおよびレポーター遺伝子アッセイにおけるヒストンの改変の分析.

主要な成果:

  • 遺伝子ターゲティングとヒストンの改変を可能にするCtBP複合体が特定されました.
  • CtBPの阻害により,E-cadherinプロモーターのヒストンの変化が起きました.
  • CtBPを抑制すると,E-カデリンのプロモーター活性が増加します.

結論:

  • CtBPは,調整された遺伝子ターゲティングとヒストンの修正を通じて,転写抑制を媒介する.
  • これらの発見は,CtBPが腫瘍発生に関与するメカニズムを明らかにしています.