酵母におけるフォークヘッド転写因子による延伸RNAポリメラーゼIIの調節

Antonin Morillon¹, Justin O'Sullivan, Abul Azad

¹Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK.

Science (New York, N.Y.)

|April 19, 2003

まとめ

イーストフォークヘッドの転写因子Fkh1pとFkh2pは,遺伝子転写の延長と終了を調節する. これらの要因は,メッセンジャーRNA処理と早期の転写延長を調整し,真核生物の遺伝子調節に影響を与えます.

科学分野:

分子生物学は分子生物学である.
遺伝子規制遺伝子規制
バイオケミストリーバイオケミストリー

背景:

RNAポリメラーゼII (RNAPII) による転写延長は,規制されたプロセスです.
早期の延長チェックポイントは,トランスクリプションと前メッセンジャーRNA (pre-mRNA) 処理をリンクします.
RNAPIIのカルボキシ末端領域 (CTD) は,転写中にリン酸化される.

研究の目的:

トランスクリプションの調節における酵母分岐骨の転写因子Fkh1pおよびFkh2pの役割を調査する.
Fkh1pとFkh2pがRNAPII延長とプレ-mRNA処理にどのように影響するかを決定する.
トランスクリプションの延長とCTDのリン酸化との連携を探求する.

主な方法:

Fkh1pとFkh2pが活性遺伝子のコーディング領域と関連している.
転写の延長と終結の分析.
RNAPII CTDのセリン5およびセリン2のリン酸化のモニタリング.

主要な成果:

Fkh1pとFkh2pは,活性遺伝子のコーディング領域と関連しています.
これらの要因は,トランスクリプションの延長と終結を正反対に影響する.
Fkh1p/Fkh2p媒介のイベントは,RNAPII CTDリン酸化とセルリン-5および-2.2で調整されます.

結論:

Fkh1pとFkh2pは,プロモーターの役割を超えて,早期の転写延長とpre-mRNA処理を調整する.
この調整は,RNAPII CTDの特定のリン酸化パターンと関連しています.
この発見は,ユカリオットの遺伝子調節のための一般的なメカニズムを示唆しています.

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