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Updated: Aug 11, 2026

A Protocol for the Identification of Protein-protein Interactions Based on 15N Metabolic Labeling, Immunoprecipitation, Quantitative Mass Spectrometry and Affinity Modulation
14:44

A Protocol for the Identification of Protein-protein Interactions Based on 15N Metabolic Labeling, Immunoprecipitation, Quantitative Mass Spectrometry and Affinity Modulation

Published on: September 24, 2012

質量スペクトロメトリー,定位,H/D交換によるタンパク質-リガンド相互作用の定量化: PLIMSTEX

Mei M Zhu1, Don L Rempel, Zhaohui Du

  • 1Resource for Mass Spectrometry, Department of Chemistry, Washington University, St. Louis, Missouri 63130, USA.

Journal of the American Chemical Society
|May 2, 2003
PubMed
まとめ

私たちは,タンパク質-リガンド相互作用と溶液における構成変化を定量化するために,新しい方法である質量スペクトロメトリ,定位,H/D交換によるタンパク質-リガンド相互作用 (PLIMSTEX) を開発しました.

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The Equilibrium Binding Constant and Binding Strength

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Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
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