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関連する概念動画

Facilitated Transport01:19

Facilitated Transport

The chemical and physical properties of plasma membranes cause them to be selectively permeable. Since plasma membranes have both hydrophobic and hydrophilic regions, substances need to be able to transverse both regions. The hydrophobic area of membranes repels substances such as charged ions. Therefore, such substances need special membrane proteins to cross a membrane successfully. In facilitated transport, also known as facilitated diffusion, molecules and ions travel across a membrane via...
Facilitated Transport01:19

Facilitated Transport

The chemical and physical properties of plasma membranes cause them to be selectively permeable. Since plasma membranes have both hydrophobic and hydrophilic regions, substances need to be able to transverse both regions. The hydrophobic area of membranes repels substances such as charged ions. Therefore, such substances need special membrane proteins to cross a membrane successfully. In  facilitated transport, also known as facilitated diffusion, molecules and ions travel across a membrane via...
Multi-pass Transmembrane Proteins and β-barrels01:09

Multi-pass Transmembrane Proteins and β-barrels

In multi-pass transmembrane proteins, the polypeptide chain crosses the membrane more than once. The transmembrane polypeptide chain either forms an α-helix or β-strand structure. α-Helix containing multi-pass transmembrane proteins are ubiquitous, whereas β-strand containing ones are mainly found in gram-negative bacteria, mitochondria, and chloroplasts.
α-Helix containing multi-pass transmembrane proteins
Multi-pass transmembrane proteins such as G-protein-linked receptors (GPCRs) and...
Facilitated Transport01:19

Facilitated Transport

The chemical and physical properties of plasma membranes cause them to be selectively permeable. Since plasma membranes have both hydrophobic and hydrophilic regions, substances need to be able to transverse both regions. The hydrophobic area of membranes repels substances such as charged ions. Therefore, such substances need special membrane proteins to cross a membrane successfully. In  facilitated transport, also known as facilitated diffusion, molecules and ions travel across a membrane via...
Cystic Fibrosis: Pathogenesis01:23

Cystic Fibrosis: Pathogenesis

Cystic fibrosis (CF), an autosomal recessive disorder, significantly affects the function of exocrine glands. This genetically inherited disease is characterized by the production of thick and sticky mucus, which can severely affect various organs and systems in the body.
CF is primarily caused by a genetic mutation in a chromosome 7 gene coding for the cystic fibrosis transmembrane conductance regulator (CFTR) protein. The most common gene mutation leading to CF is the ΔF508 mutation, but...
Facilitated Diffusion01:16

Facilitated Diffusion

The plasma membrane, a critical structure in cellular biology, houses an array of transporters, or carrier proteins, interspersed within its lipid bilayer. These proteins play a crucial role in solute transport through facilitated diffusion, a form of passive diffusion that uses transporters to move the molecules across the membrane.
In this process, substrates such as organic compounds and ions interact with a transporter on one side, triggering conformational changes in proteins that enable...

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関連する実験動画

Updated: Jul 8, 2026

In Vitro Analysis of PDZ-dependent CFTR Macromolecular Signaling Complexes
10:05

In Vitro Analysis of PDZ-dependent CFTR Macromolecular Signaling Complexes

Published on: August 13, 2012

CFTRによって形成される多機能水性チャンネル.

H Hasegawa1, W Skach, O Baker

  • 1Department of Medicine, University of California, San Francisco 94143-0532.

Science (New York, N.Y.)
|November 27, 1992
PubMed
まとめ
この要約は機械生成です。

胞性線維症のトランスメブラン伝導性調節器 (CFTR) タンパク質は,アニオン,水,尿素のcAMP刺激による輸送を促進します. この研究は,CFTRには機能的な水性毛穴が含まれていることを確認し,イオンチャネルを通る水の移動の証拠を提供します.

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Purification of the Cystic Fibrosis Transmembrane Conductance Regulator Protein Expressed in Saccharomyces cerevisiae
15:12

Purification of the Cystic Fibrosis Transmembrane Conductance Regulator Protein Expressed in Saccharomyces cerevisiae

Published on: May 10, 2014

Functional Reconstitution and Channel Activity Measurements of Purified Wildtype and Mutant CFTR Protein
09:59

Functional Reconstitution and Channel Activity Measurements of Purified Wildtype and Mutant CFTR Protein

Published on: March 9, 2015

関連する実験動画

Last Updated: Jul 8, 2026

In Vitro Analysis of PDZ-dependent CFTR Macromolecular Signaling Complexes
10:05

In Vitro Analysis of PDZ-dependent CFTR Macromolecular Signaling Complexes

Published on: August 13, 2012

Purification of the Cystic Fibrosis Transmembrane Conductance Regulator Protein Expressed in Saccharomyces cerevisiae
15:12

Purification of the Cystic Fibrosis Transmembrane Conductance Regulator Protein Expressed in Saccharomyces cerevisiae

Published on: May 10, 2014

Functional Reconstitution and Channel Activity Measurements of Purified Wildtype and Mutant CFTR Protein
09:59

Functional Reconstitution and Channel Activity Measurements of Purified Wildtype and Mutant CFTR Protein

Published on: March 9, 2015

科学分野:

  • 分子生物学は分子生物学である.
  • 生理学 生理学とは
  • バイオフィジックス 生物物理学

背景:

  • システィック線維症遺伝子産物 (CFTR) は,cAMP刺激によるイオンチャネルである.
  • 細胞内プロセスの調節におけるその役割と水中輸送のためのその可能性は完全に理解されていません.

研究 の 目的:

  • CFTR分子に機能的な水性経路があるかどうかを判断する.
  • CFTRを通じてアニオン,水,尿素の輸送を調査する.

主な方法:

  • CFTR.を発現するXenopusの卵細胞を用いて輸送アッセイを行った.
  • 測定には,アニオン,水,尿素の透過性が含まれていました.
  • cAMPアゴニストとCl-チャネルブロッカーの効果を評価した.

主要な成果:

  • cAMPの刺激により,CFTR発現性オオサイトにおける Cl-伝導性が顕著になり,水浸透性が増加した.
  • 水の浸透性はアニオン組成に依存し,Cl-チャネルブロッカーによって抑制された.
  • CFTRは,cAMPによって刺激された尿素の輸送を証明したが,砂糖は示さなかった.

結論:

  • CFTRは,アニオン,水,尿素などの小さな溶液を運ぶことができるcAMP刺激の水性毛穴として機能します.
  • これらの発見は,イオンチャネルを通した水の移動の機能的な証拠を提供します.
  • この研究は,CFTRタンパク質の輸送能力を明らかにしています.