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Updated: Jul 11, 2026

Fluorescence Based Primer Extension Technique to Determine Transcriptional Starting Points and Cleavage Sites of RNases In Vivo
10:51

Fluorescence Based Primer Extension Technique to Determine Transcriptional Starting Points and Cleavage Sites of RNases In Vivo

Published on: October 31, 2014

E. coliにおける5'-末端塩基ペアリングによるRNase E媒介RNA分解の制御

P Bouvet1, J G Belasco

  • 1Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115.

Nature
|December 3, 1992
PubMed
まとめ

エンドヌクレアースであるRNase Eは,バクテリアのメッセンジャーRNA (mRNA) の分解に不可欠です. 5'端にペア化されていない核酸を持つRNAを好ましく分割し,Escherichia coliにおけるmRNA分解率に影響を与えます.

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Identification of RNA Fragments Resulting from Enzymatic Degradation using MALDI-TOF Mass Spectrometry09:20

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RNA Stability

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RNA Stability01:53

RNA Stability

Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
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Types of RNA01:20

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Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
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