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定期的な身体活動により,冠動脈疾患の患者では,内皮酸化窒素合成酵素のリン酸化を増加させることで,内皮機能が改善されます
1Heart Center, Department of Cardiology, University of Leipzig, Germany. hamr@medizin.uni-leipzig.de
Circulation
|June 18, 2003
まとめ
運動トレーニングは,安定した冠動脈疾患 (CAD) の患者の血管機能を改善します. この改善は,内皮の酸化窒素合成酵素 (eNOS) の活性が増加し,血流の改善につながっています.
科学分野:
- 心血管医学 心血管医学
- 運動生理学 運動生理学
- 分子生物学は分子生物学である.
背景:
- 安定した冠動脈疾患 (CAD) の患者は,動脈内皮機能に肯定的な影響を及ぼす運動トレーニングの恩恵を受けます.
- 内皮 NO 合成酵素 (eNOS) からの酸化窒素 (NO) は,動脈硬化症の予防に不可欠です.
- この研究では,運動トレーニングが内皮機能,eNOS発現,および左内乳動脈 (LIMA) での Aktに依存するeNOSリン酸化に及ぼす影響を調査しています.
研究 の 目的:
- 安定したCAD患者の内皮機能に運動トレーニングがどのように影響するかを調べる.
- LIMAにおける運動訓練,eNOS発現,およびAkt依存のeNOSリン酸化との関係を評価する.
主な方法:
- 17人の研修患者と18人の対照患者には,アセチルコリンへの反応として,内皮に依存する血管拡張と平均ピークフロー速度 (APV) の侵入的測定が行われました.
- LIMAの組織は,ウェスタン・ブロットとRT-PCRを用いて,eNOS発現,フォスフォ-eNOS-Ser1177,Akt,およびフォスフォ-Aktを分析した.
- 測定はベースラインと4週間の運動介入後に行われました.
主要な成果:
- 運動トレーニングは,アセチルコリンに反応したLIMA血管拡張を56%大幅に増加させた.
- 訓練を受けた患者は,eNOSのタンパク質発現が2倍,eNOSのSer1177-phosphorylationが4倍増加したことを示しました.
- Aktのリン酸化,フォスフォ-eNOSレベル,およびAPVの変化との間の正の相関が確認されました.
結論:
- 運動トレーニングは,安定したCADのアゴニスト媒介,内皮依存性血管拡張を高めます.
- 観察された血管拡張の改善は,セール1177.7のeNOSのシャーストレスの誘発による,アクト依存のリン酸化と密接に関連しています.
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