ヒトの単一の骨髄幹細胞から,血液形成微環境と血液形成幹細胞の形成

S Huang¹, L W Terstappen

¹Becton Dickinson Immunocytometry Systems, San Jose, California 95131.

Nature

|December 24, 1992

まとめ

研究者は,ヒト胎児の骨髄幹細胞の2つの新しいサブセットを特定しました. 一つのサブセットは,血液細胞を再構成し,より原始的なサブセットは,血液細胞の微分化を可能にする,骨髄の微環境をサポートします.

科学分野:

血液学ヘマトロジ
幹細胞生物学幹細胞生物学
免疫学免疫学とは

背景:

造血幹細胞 (HSC) は自己再生と分化能力を持っています.
CD34発現はヒトのHSC濃縮のマーカーですが,ほとんどのCD34+細胞は系統にコミットされています.
真の多能性HSCサブセットの特徴づけは,血液形成を理解するために重要である.

研究の目的:

人間の胎児の骨髄内の異なる多能幹細胞サブセットを特定し,特徴づけること.
これらのサブセットの微分化可能性を調査する.
造血細胞とその微小環境の両方を再構成できる単一の細胞を特定する.

主な方法:

細胞表面マーカー分析のためのフローサイトメトリ (CD34,HLA-DR,CD38).
系統の可能性を評価するための in vitro 微分化検査.
溶解能力を評価するための機能的測定法.

主要な成果:

CD34+,HLA-DR+,CD38-サブセットの識別,すべての血液生成系に差異化しています.
より原始的なCD34+,HLA-DR-,CD38-サブセットを特定し,血液形成前駆体およびサポート性ストロマ細胞を生成することができる.
原始的なサブセットが血液生成細胞と骨髄のマイクロ環境の両方を再構成できることを実証.

結論:

人間の胎児の骨髄には,2つの異なる多能幹細胞集団が存在する.
原始的なHSCのサブセットは,必要なマイクロ環境を提供することによって,血液形成をサポートします.
この研究は,血液形成系全体とそのサポートニッチを再構成する能力を持つ単一の細胞の最初の識別を報告しています.

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