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関連する概念動画

Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Introduction to Fibroblasts01:09

Introduction to Fibroblasts

Rudolph Virchow discovered spindle-shaped cells called fibroblasts in 1858. Inactive fibroblasts, called fibrocytes, become activated by various stimuli, such as growth factors and inflammatory cytokines. Activated fibroblasts play a crucial role in wound healing, inflammation, formation of new blood vessels, and cancer progression. Uncontrolled activation of fibroblasts results in fibrosis, the excess deposition of fibrous tissue, which can lead to scarring and affect normal organs. This...
Polymer Classification: Architecture01:14

Polymer Classification: Architecture

Polymers are classified as linear or branched on the basis of their chain architecture. The polymer chains in linear polymers have a long chain-like structure with minimal to no branching at all. Even if a polymer features large substituent groups on the monomer, which appear as branches to the skeleton, it is not considered a branched polymer. A branched polymer contains secondary polymer chains that arise from the main polymer chain. The branching occurs when the polymer growth shifts from...
Fibril-associated Collagen01:11

Fibril-associated Collagen

Fibril-associated collagens are a type of collagens present in the extracellular matrix with interrupted triple helices or FACIT (Fibril-associated collagens interrupted triple-helices). FACIT help connect and attach the collagen fibrils with each other as well as with other proteins of the extracellular matrix.
For example, the type II collagen fibrils in cartilage have covalently bound type IX fibril-associated collagens at regular intervals. Other types of fibril-associated collagens are...
Multipotency and Niche of Bulge Stem Cell01:06

Multipotency and Niche of Bulge Stem Cell

A hair follicle or HF is a small part of the skin that produces the hair shaft. Paul Gerson Unna was the first to observe a bulge in the human hair follicle's outer root sheath (ORS). The bulge is present between the sebaceous gland and the arrector pili muscle and is the niche for hair follicle stem cells (HFSCs). The bulge is also a niche for melanocyte stem cells, and their loss results in graying of hair. The HFSCs express Sox9 and Lhx2, which help them maintain stemness and prevent...

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枝分かれ形質変異におけるフィブロネクチンの必要性

Takayoshi Sakai1, Melinda Larsen, Kenneth M Yamada

  • 1Craniofacial Developmental Biology and Regeneration Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland 20892-4370, USA.

Nature
|June 20, 2003
PubMed
まとめ

フィブロネクチンは,胚の臓器発達に不可欠であり,枝分かれ形態変異の間に裂け目の形成を可能にします. この細胞外マトリックスタンパク質は,細胞-細胞から細胞-マトリックス結合への移行を促進し,臓器構造に不可欠です.

科学分野:

  • 発達生物学 発達生物学とは
  • 細胞生物学 細胞生物学
  • 細胞外マトリックス生物学 細胞外マトリックス生物学

背景:

  • オルガノゲネシスは,繰り返し裂け目と芽の形成を含む上皮の分岐形態変異に依存しています.
  • これらの構造的変化,特に裂け目の形成を駆動する正確な分子機構は,ほとんど不明のままである.
  • 以前の仮説では,局所的な調節タンパク質が枝分かれパターンを導くことに関与することを示唆していた.

研究 の 目的:

  • 細胞外マトリックスタンパク質が,上皮の分岐を誘発する役割を調査する.
  • 枝分かれ形質変異 (branching morphogenesis) 中の裂け目形成におけるフィブロネクチンの特定の機能を決定する.
  • フィブロネクチンが上皮組織構造に影響を与える分子メカニズムを解明する.

主な方法:

  • 発育中の唾液腺におけるフィブロネクチンメッセンジャーRNAとタンパク質の局所化と時間表現分析.
  • 小型の干渉RNAを用いてフィブロネクチンレベルを低下させ,フィブロネクチンとインテグリンの抗体阻害を行う機能検査.
  • 外因性フィブロネクチンの分岐への影響を評価するための実験.
  • 培養されたヒト唾液上皮細胞を用いたインビトロ研究で,フィブロネクチン誘発の粘着変化を調べた.

主要な成果:

さらに関連する動画

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関連する実験動画

Last Updated: Jun 13, 2026

Scanning Electron Microscopy (SEM) Protocols for Problematic Plant, Oomycete, and Fungal Samples
10:57

Scanning Electron Microscopy (SEM) Protocols for Problematic Plant, Oomycete, and Fungal Samples

Published on: February 3, 2017

Biological Compatibility Profile on Biomaterials for Bone Regeneration
10:28

Biological Compatibility Profile on Biomaterials for Bone Regeneration

Published on: November 16, 2018

Microfluidic Tools for Probing Fungal-Microbial Interactions at the Cellular Level
08:19

Microfluidic Tools for Probing Fungal-Microbial Interactions at the Cellular Level

Published on: June 23, 2022

  • フィブロネクチンの発現は,裂け目領域の発達において一時的で焦点的であり,カデリンの喪失と相関していた.
  • フィブロネクチンの抑制 (siRNAまたは抗体経由) は,唾液腺,肺,腎臓の裂け目形成と分岐を抑制しました.
  • 外因性フィブロネクチンの添加は,裂け目の形成と分岐を促した.
  • 繊維性フィブロネクチンは,培養された上皮細胞における細胞マトリックス粘着とカデリン喪失を誘発した.

結論:

  • フィブロネクチンは,上皮の分岐形態変異の開始時に裂け目形成に不可欠です.
  • フィブロネクチンは,細胞-細胞結合から細胞-マトリックス結合への重要な移行を媒介し,発達中の臓器の構造的変化を促します.
  • これらの発見は,複雑な臓器構造の発達におけるフィブロネクチンの不可欠な役割を強調しています.