血小板活性化因子アセチルヒドロラゼは,主に低密度リポプロテインの電子負子分数と関連しています

Sonia Benítez¹, José Luis Sánchez-Quesada, Vicent Ribas

¹Servei de Bioquímica and Institut de Recerca, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain.

Circulation

|June 25, 2003

まとめ

電子負のLDL (LDL(-)) には,血小板活性化因子アセチルヒドロラーゼ (PAF-AH) の活性が著しく高い. LDLのPAF-AHの増加は,内皮細胞に対する炎症効果に貢献する可能性がある.

科学分野:

バイオケミストリーバイオケミストリー
脂質代謝についてです.
心血管研究循環器科の研究

背景:

電子負のLDL (LDL(-)) は,改変した脂質タンパク質分数である.
LDL(-) は,内皮細胞から炎症性サイトカイン (IL-8,MCP-1) の放出を誘導する.

研究の目的:

血小板活性化因子アセチルヒドロラーゼ (PAF-AH) と電子負のLDL (LDL(-) の関連性を調査する.
PAF-AHの活性がLDL ((-) と非電子負のLDL (LDL ((+)) の間で異なっているかどうかを判断する.

主な方法:

ノルモリペミアおよび高コレステロールの被験者からのLDL (LDL) とLDL (LDL) のPAF-AH活性評価.
PAF-AHを特定するために,ウエスタン・ブロット分析とSDS-PAGEを用いた.
Nondenaturing polyacrylamide gradient gel electrophoresisを使用して,PAF-AHがLDLと結合することを評価しました.

主要な成果:

PAF-AHは主にLDL (((-) と関連しており,LDL (((+) に比べて5倍以上の活性を示した.
ウェスタン・ブロットは,LDLのPAF-AHの存在を確認したが,LDLの存在は確認されなかった.
PAF-AH産物である非精製脂肪酸は,LDLで上昇していました.

結論:

LDLのPAF-AHの活性が上昇したのが重要な発見である.
この高いPAF-AH活性が,内皮細胞におけるLDLの炎症性特性を説明するかもしれない.

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