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Updated: Jul 17, 2026

A Fluorescence-based Assay of Phospholipid Scramblase Activity
09:52

A Fluorescence-based Assay of Phospholipid Scramblase Activity

Published on: September 20, 2016

合成PSスクランブラーゼを用いたフォスファティディルセリン (PS) のフリップフロップとトロビン活性化を促進した

J Middleton Boon1, Timothy N Lambert, Adam L Sisson

  • 1Department of Chemistry and Biochemistry and the Walther Cancer Research Center, University of Notre Dame, Notre Dame, Indiana 46556, USA.

Journal of the American Chemical Society
|July 3, 2003
PubMed
まとめ

研究者らは,フォスファティディルセリン (PS) スクランブラゼとして作用するカチオン性ステロイドを合成し,膜にわたってPSの転位を促進し,細胞表面のPSレベルを上昇させた. この合成PSスクランブラーゼは,赤血球におけるトロンビン生成を著しく強化する.

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Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies10:01

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Carboxylic Acids to Acid Chlorides01:18

Carboxylic Acids to Acid Chlorides

Carboxylic acids react with SOCl2 or PCl5 to form acid chlorides. Amongst the carboxylic acid derivatives, acid chlorides are the most reactive and synthetically important derivatives. They are useful reagents for Friedel–Crafts acylation of some aromatic compounds.
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Acid Halides to Carboxylic Acids: Hydrolysis

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As shown below, the mechanism involves a nucleophilic attack by water at the carbonyl carbon to form a tetrahedral intermediate. This is followed by the reformation of the carbon–oxygen π bond along with the departure of a halide ion. A final proton transfer step yields carboxylic acid...
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Membrane Asymmetry Regulating Transporters

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Flippase
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