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Updated: Jul 6, 2026

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism
11:37

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism

Published on: July 28, 2017

オリゴヌクレオシドフォスフォロチオ酸塩のステレオ制御合成のためのオクサザフォスフォリジンのアプローチ

Natsuhisa Oka1, Takeshi Wada, Kazuhiko Saigo

  • 1Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Bioscience Building 702, Kashiwa, Chiba 277-8562, Japan.

Journal of the American Chemical Society
|July 3, 2003
PubMed
まとめ

この研究では,オキサザフォスホリジンの単体を使用したフォスフォロチオ酸オリゴデオキシリボヌクレオシド (PS-ODNs) のステレオ制御合成のための新しい方法について詳細に述べています. このアプローチは,高いダイアステレオ選択性を達成し,固体相合成などのアプリケーションのための純粋な (R(p)) -および (S(p)) -PS-ODNsの生産を可能にします.

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Preparation of Alkynes: Alkylation Reaction

Introduction
Alkylation of terminal alkynes with primary alkyl halides in the presence of a strong base like sodium amide is one of the common methods for the synthesis of longer carbon-chain alkynes. For example, treatment of 1-propyne with sodium amide followed by reaction with ethyl bromide yields 2-pentyne.
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Preparation of 1° Amines: Gabriel Synthesis

Direct alkylation is not a suitable method for synthesizing amines because it produces polyalkylated products. Gabriel synthesis is the most preferred method to exclusively make primary amines. The method uses phthalimide, which contains a protected form of nitrogen that participates in alkylation only once to predominantly give primary amines.
Strong bases like NaOH or KOH deprotonate the phthalimide to form the corresponding anion, which acts as a nucleophile. Further, the anion attacks an...
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Preparation of 1° Amines: Hofmann and Curtius Rearrangement Mechanism

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