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関連する概念動画

Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
M-Cdk Drives Transition Into Mitosis02:15

M-Cdk Drives Transition Into Mitosis

Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Anaphase Promoting Complex00:50

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
Inhibition of CDK Activity02:34

Inhibition of CDK Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
M-Cdk Drives Transition Into Mitosis02:15

M-Cdk Drives Transition Into Mitosis

Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Anaphase Promoting Complex00:50

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...

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関連する実験動画

Updated: Jun 27, 2026

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
12:02

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

Published on: June 6, 2017

Xpd/Ercc2は,CAKの活性とミトスの進行を調節する.

Jian Chen1, Stéphane Larochelle, Xiaoming Li

  • 1Present address: Cell Biology Program, Memorial Sloan-Kettering Cancer Center, New York, New York 10021, USA.

Nature
|July 11, 2003
PubMed
まとめ

ドロソフィラのTFIIH成分XpdはCdk7キナーゼの活性を否定的に調節し,細胞サイクル進行に影響を与える. ミトーシスにおけるXpdのダウンレギュレーションは,細胞分裂のためのCdk7の活動を強化し,転写を静止させます.

科学分野:

  • 細胞生物学 細胞生物学
  • 分子生物学は分子生物学である.
  • 遺伝学 遺伝学とは

背景:

  • 一般転写因子IIH (TFIIH) は,転写とDNA修復に不可欠な複数のサブユニット複合体です.
  • TFIIHには,Cdk7,サイクリンH,MAT1を含むCdk活性化キナーゼ (CAK) サブコンプレックスが含まれ,これはサイクリン依存キナーゼ (Cdks) を活性化します.
  • Cdk7は,TFIIHのキナーゼサブユニットとして,RNAポリメラーゼIIや他のCdkをリン酸化し,転写と細胞サイクル進行を調節する.

研究 の 目的:

  • Cdk7/CAKの活動を調節するTFIIHコンポーネントXpdの役割を調査する.
  • Xpdが細胞サイクル進行とミトーシスイベントにどのように影響するかを決定する.
  • ミトーシス中のXpd,CAK活動,および転写調節の相互作用を解明する.

主な方法:

  • ドロソフィラをモデル生物として利用した.
  • Cdk7/CAK活動への影響を観察するために,Xpdレベルを操作した.
  • 評価されたCdk T-ループのリン酸化,ミトーシス進行,細胞増殖,および転写活動.

主要な成果:

  • 過剰なXpdはCAK活性を定位し,Cdk T-ループのリン酸化,ミトスの欠陥,および致死性を低下させます.

さらに関連する動画

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
08:33

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

A Non-random Mouse Model for Pharmacological Reactivation of Mecp2 on the Inactive X Chromosome
08:27

A Non-random Mouse Model for Pharmacological Reactivation of Mecp2 on the Inactive X Chromosome

Published on: May 22, 2019

関連する実験動画

Last Updated: Jun 27, 2026

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
12:02

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

Published on: June 6, 2017

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
08:33

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

A Non-random Mouse Model for Pharmacological Reactivation of Mecp2 on the Inactive X Chromosome
08:27

A Non-random Mouse Model for Pharmacological Reactivation of Mecp2 on the Inactive X Chromosome

Published on: May 22, 2019

  • 減少したXpdレベルは,CAKの活動を高め,細胞増殖を促進します.
  • Xpdはミトーシスの初期にダウンレギュレーションされ,高いCdk1活性と一致します.

    • 結論:

    • ドロソフィラXpdはCdk7/CAKの活性を否定的に調節し,それによって細胞サイクル進行を制御する.
    • ミトーシス中のXpdのダウンレギュレーションは,CAKの活性をアップレギュレーションし,ミトーシスの進行を促進します.
    • Xpdのミトスのダウンレギュレーションは,細胞分裂中の基礎転写を静止する重要なメカニズムです.