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関連する概念動画

Cell Migration01:09

Cell Migration

Cell migration, the process by which cells move from one location to another, is essential for the proper development and viability of organisms throughout their life. When cells are not able to migrate properly to their ordained locations, various disorders may occur. For example, disruption in cell migration causes chronic inflammatory diseases such as arthritis.
Cell Migration01:19

Cell Migration

Cell migration is a process by which the cells move from one location to another, playing an essential role in embryological development, repair and regeneration, immune response, and metastasis. Cells migrate in response to chemical or mechanical signals generated by specific organs or tissues. The overall mechanism includes three steps - polarization, protrusion, and release. Polarization involves the formation of a distinct cell front and rear, which determines the direction of movement.
Cell Motility through Blebbing01:16

Cell Motility through Blebbing

Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
In multicellular...
Role of Myosin in Cell Migration01:18

Role of Myosin in Cell Migration

Myosins are multimeric motor proteins involved in various cellular processes such as migration, adhesion, and proliferation. Myosin II is the most common type in animal cells, which binds and cross-links actin filaments.
Myosin II  is a hexamer comprising two heavy chains with globular heads and coiled-coil tails, two regulatory light chains, and two essential light chains. The ATPase sites on the myosin heads hydrolyze ATP, and the released phosphate generates the force for contraction. It is...
Chemotaxis and Direction of Cell Migration01:21

Chemotaxis and Direction of Cell Migration

Cells can detect chemical cues in their environment and reorganize the cytoskeleton to migrate toward them or away from them. This directional migration, called chemotaxis, is essential during embryogenesis and development, immune response, tissue repair and regeneration, and reproduction. These chemical cues can either attract or repel the cell's movement. For example, axon development is determined by a combination of chemoattractants and chemorepellents that direct the growing axon towards...
Cytoskeletal Coordination in Cell Migration01:32

Cytoskeletal Coordination in Cell Migration

A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker proteins that...

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関連する実験動画

Updated: Jul 9, 2026

Analyzing In Vivo Cell Migration using Cell Transplantations and Time-lapse Imaging in Zebrafish Embryos
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Analyzing In Vivo Cell Migration using Cell Transplantations and Time-lapse Imaging in Zebrafish Embryos

Published on: April 29, 2016

WAVE2は,指向された細胞移動と心血管の発達のために必要です.

Daisuke Yamazaki1, Shiro Suetsugu, Hiroaki Miki

  • 1Department of Biochemistry, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Mianato-ku, Tokyo 108-8639, Japan.

Nature
|July 25, 2003
PubMed
まとめ

WAVE2タンパク質は,胚の発達中の細胞移動と血管形成に不可欠です. マウスの体内でのその欠如は,発達障害や血管新生障害を引き起こし,WAVE2を強調した.

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Characterizing Cell Migration Within Three-dimensional In Vitro Wound Environments
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Characterizing Cell Migration Within Three-dimensional In Vitro Wound Environments

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Deep and Spatially Controlled Volume Ablations using a Two-Photon Microscope in the Zebrafish Gastrula
09:50

Deep and Spatially Controlled Volume Ablations using a Two-Photon Microscope in the Zebrafish Gastrula

Published on: July 15, 2021

関連する実験動画

Last Updated: Jul 9, 2026

Analyzing In Vivo Cell Migration using Cell Transplantations and Time-lapse Imaging in Zebrafish Embryos
11:39

Analyzing In Vivo Cell Migration using Cell Transplantations and Time-lapse Imaging in Zebrafish Embryos

Published on: April 29, 2016

Characterizing Cell Migration Within Three-dimensional In Vitro Wound Environments
06:10

Characterizing Cell Migration Within Three-dimensional In Vitro Wound Environments

Published on: August 16, 2017

Deep and Spatially Controlled Volume Ablations using a Two-Photon Microscope in the Zebrafish Gastrula
09:50

Deep and Spatially Controlled Volume Ablations using a Two-Photon Microscope in the Zebrafish Gastrula

Published on: July 15, 2021

科学分野:

  • 発達生物学 発達生物学について
  • 細胞生物学 細胞生物学
  • 分子生物学は分子生物学である.

背景:

  • 形質変異に欠かせない細胞の運動性は,ウィスコット・オルドリッヒ症候群のタンパク質と関連しているWAVE2のようなタンパク質によって調節されます.
  • WAVE2は,細胞移動の重要な構成要素であるRac誘発の膜乱れに不可欠です.

研究 の 目的:

  • 胚形成中のWAVE2の生理学的機能を調査する.
  • 細胞の移動と血管新生におけるWAVE2の役割を決定する.

主な方法:

  • マウスにおけるWAVE2の遺伝子破壊 (WAVE2-/-).
  • 胚の発達,血管化,および内皮細胞の行動の分析.
  • 血管内皮成長因子に対する反応として,細胞の極性およびラメリポディアの形成の評価.

主要な成果:

  • WAVE2は,胚形成中に主として血管内皮細胞で発現した.
  • WAVE2-/-胚は,出血と10日頃の胚死亡率を示した.
  • 血管新生は影響を受けなかったが,内皮細胞の芽生えと枝分かれが減少したため,血管新生は損なわれた.
  • WAVE2欠乏症は,正常な細胞の極性形成にもかかわらず,内皮細胞のラメリポディア形成を深刻に阻害しました.

結論:

  • WAVE2は,適切な細胞移動,特に内皮細胞におけるラメリポディアの形成に不可欠です.
  • WAVE2 調節されたアクチン再編成は,有効な血管新生 in vivo に不可欠です.
  • WAVE2の障害は,血管の発達障害と胚の死亡につながる.