Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

The DNA Helix01:16

The DNA Helix

129.0K
Overview
129.0K
Proofreading01:43

Proofreading

52.0K
Overview
52.0K
DNA as a Genetic Template02:05

DNA as a Genetic Template

22.1K
Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...
22.1K
Chromosome Replication02:31

Chromosome Replication

8.8K
Before a cell can divide, it must accurately replicate all of its chromosomes, including the DNA and its associated histone and non-histone proteins.  This process begins at numerous origins of replication during the S phase of the cell cycle in each of a cell’s chromosomes simultaneously. Certain nucleotides can act as origins of replication, but these sequences are not well defined - especially in complex, multi-cellular, eukaryotic species. The length of DNA that spans an origin...
8.8K
Lagging Strand Synthesis01:59

Lagging Strand Synthesis

38.0K
During replication, the complementary strands in double-stranded DNA are synthesized at different rates. Replication first begins on the leading strand. Replication starts later, occurs more slowly, and proceeds discontinuously on the lagging strand.
There are several major differences between synthesis of the leading strand and synthesis of the lagging strand. 1) Leading strand synthesis happens in the direction of replication fork opening, whereas lagging strand synthesis happens in the...
38.0K
The DNA Helix01:07

The DNA Helix

19.8K
Deoxyribonucleic acid, or DNA, is the genetic material responsible for passing traits from generation to generation in all organisms and most viruses. DNA is composed of two strands of nucleotides that wind around each other to form a spring-like structure called a double helix. However, the double helix is not perfectly symmetrical. Instead, there are regularly occurring grooves in the structure. The major groove occurs where the sugar-phosphate backbones are relatively far apart. This space...
19.8K

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Using sequence-derived microbiome information from bulk tank milk samples to assess the influence of post-milking teat disinfection in New Zealand dairy herds.

Animal microbiome·2026
Same author

Enhanced exosome biomanufacturing, precision isolation, and advanced engineering: toward mechanistic regulation and biomaterial integration for osteoarthritis therapy.

Journal of nanobiotechnology·2026
Same author

Epithelial SLC39A1 prevents acute lung injury through zinc-mediated transcriptional activation of autophagy in male mice.

Nature communications·2026
Same author

Activation of Escherichia coli RecA* in recA730 lexA<sup>+</sup> and recA730 lexA(Def) strain backgrounds.

DNA repair·2026
Same author

Acinetobacter baumannii encodes multiple mutagenically and biochemically active DNA polymerase V variants.

Nucleic acids research·2026
Same author

Declining prevalence of multidrug-resistant but comparatively high quinolone-resistant tuberculosis in Heilongjiang Province, China: 2013-2022 trend.

BMC infectious diseases·2026

関連する実験動画

Updated: Apr 28, 2026

Visualizing Single-molecule DNA Replication with Fluorescence Microscopy
15:57

Visualizing Single-molecule DNA Replication with Fluorescence Microscopy

Published on: October 9, 2009

25.4K

原子解像度でシスシンチミンダイマーの複製

Hong Ling1, François Boudsocq, Brian S Plosky

  • 1Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.

Nature
|August 9, 2003
PubMed
まとめ

紫外線は,サイクロブタンピリミジンジマー (CPD) によるDNA損傷を引き起こす. Y族のDNAポリメラーゼエタ (pol eta) はこれらの病変を回避し,その構造は,考古学的な同類であるDpo4を用いて解明されました.

科学分野:

  • 分子生物学は分子生物学である.
  • バイオケミストリー バイオケミストリー
  • 遺伝学 遺伝学とは

背景:

  • 紫外線 (UV) 放射線はDNAの損傷を誘導し,主にシスシンサイクロブータンピリミジンジマー (CPD) を形成します.
  • CPDは,高精度ポリメラーゼによるDNA複製を阻害し,特殊なバイパスメカニズムを必要とします.
  • DNAポリメラーゼエタ (POLH) をコードする遺伝子の欠陥は,極端な太陽感受性および皮膚がんの傾向を引き起こす病気であるキセロダーマ・ピグメンツウムに関連しています.

研究 の 目的:

  • YファミリーのDNAポリメラーゼエタがサイクロブータンピリミジンジマー (CPD) をバイパスする方法の構造的基礎を解明する.
  • 紫外線によるDNA損傷のDNA修復と複製バイパスのメカニズムを理解する.

主な方法:

  • ポリエタの古代の同質体であるDPo4の2つの結晶構造が,CPDを含むDNAとの複合体で決定されました.
  • Dpo4,CPD,および入ってくる核酸の相互作用を分析した.

主要な成果:

  • CPDの3'チミンは,ワトソン・クリックの塩基対をデデオキシATPでテンプレートした.
  • CPDの5'チミンは,シンコンフォーメーションでデデオキシATPを持つHoogsteen塩基ペアをテンプレートした.

さらに関連する動画

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography
14:56

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography

Published on: May 20, 2022

3.3K
Single-Molecule Real-Time Visualization of DNA Unwinding by CMG Helicase
07:37

Single-Molecule Real-Time Visualization of DNA Unwinding by CMG Helicase

Published on: September 27, 2024

2.2K

関連する実験動画

Last Updated: Apr 28, 2026

Visualizing Single-molecule DNA Replication with Fluorescence Microscopy
15:57

Visualizing Single-molecule DNA Replication with Fluorescence Microscopy

Published on: October 9, 2009

25.4K
Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography
14:56

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography

Published on: May 20, 2022

3.3K
Single-Molecule Real-Time Visualization of DNA Unwinding by CMG Helicase
07:37

Single-Molecule Real-Time Visualization of DNA Unwinding by CMG Helicase

Published on: September 27, 2024

2.2K
  • Dpo4の活性部位は,これらの異常なDNA構造をカタリシスに適合させる.

    • 結論:

    • Dpo4の構造は,YファミリーポリメラーゼによるCPDバイパスメカニズムについての洞察を提供します.
    • ポリタ-CPD複合体のモデルでは,効率的なCPDバイパスを可能にするユニークな構造特性が示唆されています.
    • ポリエタの機能を理解することは,DNA修復を理解し,UV誘発型変異を防ぐために極めて重要です.