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誘導可能な酸化窒素合成酵素の複雑性:細胞源が,毒性対効果を決定する
Betty Y Poon1, Eko Raharjo, Kamala D Patel
1Department of Physiology and Biophysics, Cardiovascular, University of Calgary, Alberta, Canada.
Circulation
|August 20, 2003
まとめ
心筋筋細胞における誘導性酸化窒素合成酵素 (iNOS) は,セプシス中のベータ-アドレネルジック反応に有益である. しかし,中性粒子の由来のiNOSは,筋細胞に損傷を与え,源がiNOS効果を決定することを示します.
科学分野:
- 心血管生理学 心血管の生理学
- 免疫学 免疫学とは
- 分子生物学は分子生物学である.
背景:
- 誘導性酸化窒素合成酵素 (iNOS) は,結血症において二重の役割を果たしている.
- 心臓のミオサイトと中性粒子を中心に,心臓におけるiNOSの影響を理解します.
研究 の 目的:
- セプシスにおける心筋筋細胞と中性粒子の異なった機能的効果を研究する.
- 細胞源がiNOSの活動をどのように調節し,心臓機能にどのような影響を与えるかを決定する.
主な方法:
- 暴露された内毒性心筋細胞は,野生型およびiNOS欠乏性ニュートロフィールに.
- ミオサイト縮小,酸化ストレス,ベータ・アドレナジック刺激反応を評価した.
- ニュートロフィール由来の超酸化物とペロキシニトライトの定量化.
主要な成果:
- 中性粒子が誘発するiNOSは酸化ストレスを増加させ,肌細胞の縮小を阻害する.
- しかし,Myocyte iNOSは,ベータアドレネルゲン媒介による筋細胞縮小の増加を高めました.
- 超酸化物とNOの両方が中性粒子の機能不全に不可欠であるにも関わらず,ペロキシニトリートは検出されなかった.
結論:
- 細胞源は,セプシスにおけるiNOSの役割に重大な影響を及ぼします.
- Myocyte iNOSは,ベータアドレネルジック刺激に対する心臓の反応に有益である.
- 中性粒子のiNOSはセプシス中に心筋細胞に有害である.
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