COPIIコートのSNARE選択性について
Elena Mossessova1, Lincoln C Bickford, Jonathan Goldberg
1Howard Hughes Medical Institute and the Cellular Biochemistry and Biophysics Program, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA.
Cell
|August 28, 2003
まとめ
COPIIコートタンパク質複合体は,そのSec23/24サブ複合体の3つの異なる結合部位を通じて,エンドプラズミック網膜-ゴルギSNAREを認識し,特定の膀輸送と融合を保証します.
科学分野:
- 細胞生物学 細胞生物学
- 分子生物学は分子生物学である.
- タンパク質対タンパク質の相互作用
背景:
- COPII (COat Protein Complex II) 機構は,エンドプラズマ網膜 (ER) からゴルジ装置までの膀芽生えを媒介する.
- 膀輸送は,膜融合を媒介するSNAREタンパク質に依存しています.
- COPIIによるSNAREの認識を理解することは,輸送特異性を明らかにするために不可欠です.
研究 の 目的:
- 酵母 COPII Sec23/24 亜複合体の結合部位を特定し,特徴づけ,特定の ER-Golgi SNAREs を認識する責任を負う.
- COPIIがSNAREの機能的形状をどのように選択し,結合するかを決定する.
主な方法:
- Sec23/24サブコンプレックスにおける結合部位をマッピングするための生化学分析.
- SNAREモチーフとそのCOPIIとの相互作用の分析.
- COPIIバインディング中のSNAREの構成状態を調査する.
主要な成果:
- SEC23/24サブコンプレックス内の3つの異なる結合部位 (A,B,および第3部位) は,ER-Golgi SNAREs Bet1,Sed5,およびSec22のために特定されました.
- サイトAはSed5のYNNSNPFモチーフを結合し,サイトBはLxx-L/M-EモチーフとDxE信号を結合する.
- Sec22は別のサイトに結合し,COPIIは輸送のために特定のSNARE構成 (フリーv-SNARE Bet1,t-SNARE複合体Sed5) を選択します.
結論:
- COPIIコートタンパク質は,溶融性SNARE形状の特定のセレクタとして作用する.
- この認識メカニズムは,ERからGolgiまで,膀の芽生えとターゲティングの忠実性を保証します.
- この発見は,分子レベルで膀融合特異性のプログラミングに関する洞察を提供します.
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