U. maydis の a 交配型ロクスは,細胞シグナル伝達成分を指定しています
1Institut für Genbiologische Forschung Berlin GmbH, Federal Republic of Germany.
Cell
|February 7, 1992
まとめ
ウスティラゴ・メイディス菌は,新しい交配型ロカス戦略を使用しています. 細胞融合と繊維状の成長を制御する2つの遺伝子は,交配因子とフェロモン受容体をコードする.
科学分野:
- 菌類学 菌類学とは
- 遺伝学 遺伝学とは
- 分子生物学は分子生物学である.
背景:
- ユスティラゴ・メイディスの交配型ロクスは,細胞融合や dikaryotic mycelial 成長のような重要な発達過程を調節します.
- この場所には,2つの異なるアレル,a1とa2が含まれており,それぞれ異なるDNA領域サイズ (それぞれ4.5kbと8kb) が特徴ですが,同じ配列で囲まれています.
研究 の 目的:
- Ustilago maydis.の交配型決定の基礎となる遺伝的メカニズムを解明する.
- 細胞同士の認識と,その後の発達経路を制御する特定の遺伝子を特定する.
主な方法:
- 遺伝子活動をテストするために,機能的アッセイが使用されました.
- 遺伝子機能を評価するために,突然変異の分析が行われました.
- DNAシーケンシングは,交配型ロクスの遺伝子組成を決定するために使用されました.
主要な成果:
- 研究では,各ロカスアレル (a1とa2) の内にある2つの遺伝子のセットを特定した.
- 1つの遺伝子は,リポペプチド交配因子の前駆者をコードする.
- 2番目の遺伝子は,対極の交配型の細胞によって分泌されるフェロモンの受容体を指定します.
結論:
- ウスティラゴ・メイディスは,交配型決定のユニークな戦略を使用しています.
- 細胞-細胞認識の主要な決定因子は,交配型の場所内に直接コード化されています.
- この遺伝システムは,真菌の発達と繁殖を制御する.
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