Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Protein and Protein Structure02:15

Protein and Protein Structure

Proteins are one of the most abundant organic molecules in living systems and have the most diverse range of functions of all macromolecules. Proteins may be structural, regulatory, contractile, or protective. They may serve in transport, storage, or membranes; or they may be toxins or enzymes. Their structures, like their functions, vary greatly. They are all, however, amino acid polymers arranged in a linear sequence.
A protein's shape is critical to its function. For example, an enzyme can...
ATP Synthase: Structure01:18

ATP Synthase: Structure

ATP synthase or ATPase is among the most conserved proteins found in bacteria, mammals, and plants. This enzyme can catalyze a forward reaction in response to the electrochemical gradient, producing ATP from ADP and inorganic phosphate. ATP synthase can also work in a reverse direction by hydrolyzing ATP and generating an electrochemical gradient. Different forms of ATP synthases have evolved special features to meet the specific demands of the cell. Based on their specific feature, ATP...
Structure of Amines01:19

Structure of Amines

The hybridized nitrogen atom in amines possesses a lone pair of electrons and is bound to three substituents with a bond angle of around 108°, which is less than the tetrahedral angle of 109.5°. However, the C–N–H bond angle is slightly larger at 112°, with a carbon–nitrogen bond length of 147 pm. This carbon–nitrogen bond length of of amines is longer than the carbon–oxygen bond of alcohols (143 pm) but shorter than alkanes’ carbon–carbon bond (154 pm). These aspects are illustrated in Figure...
Unit Cells01:18

Unit Cells

A crystal's internal structure is an orderly array of atoms, ions, or molecules, and the details of this array significantly influence the solid's properties. In a crystal, periodically repeating 'structural motifs' - which could be atoms, molecules, or groups thereof - create a 'space lattice.' This is essentially a three-dimensional, infinite array of points, each surrounded by its neighbors in an identical way, forming the basic structure of the crystal.A 'unit cell' is a theoretical...
Imperfections in Crystal Structure: Point, Line and Plane Defects01:25

Imperfections in Crystal Structure: Point, Line and Plane Defects

A perfect crystal, in theory, has a uniform structure with the same unit cell and lattice points throughout. However, any deviation from this periodic arrangement is known as an imperfection or defect. These defects can be categorized into three types: point, line, and plane defects.Point defects occur when there is a deviation from the ideal due to missing atoms, displaced atoms, or additional atoms. These imperfections might occur due to imperfect packing during crystallization or because of...
Imperfections in Crystal Structure: Stoichiometric Point Defects01:26

Imperfections in Crystal Structure: Stoichiometric Point Defects

Schottky defects arise when some lattice points in a crystal, such as those in NaCl, remain unoccupied, creating lattice vacancies without disturbing the overall electrical neutrality of the crystal. This defect is common in ionic crystals where the positive and negative ions are similar in size, as seen in sodium chloride and cesium chloride. The presence of Schottky defects enables the crystal to conduct electricity to a small extent through an ionic mechanism. Electric fields cause nearby...

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

An evaluation of the implementation of a parent-led, games-based physical activity intervention: the Active Play at Home quasi-randomized trial.

Health education research·2018
Same author

Correction: Analysis of unstable modes distinguishes mathematical models of flagellar motion.

Journal of the Royal Society, Interface·2017
Same author

Early-life glucocorticoids programme behaviour and metabolism in adulthood in zebrafish.

The Journal of endocrinology·2016
Same author

Early-life perturbations in glucocorticoid activity impacts on the structure, function and molecular composition of the adult zebrafish (Danio rerio) heart.

Molecular and cellular endocrinology·2015
Same author

Analysis of unstable modes distinguishes mathematical models of flagellar motion.

Journal of the Royal Society, Interface·2015
Same author

Physiological roles of glucocorticoids during early embryonic development of the zebrafish (Danio rerio).

The Journal of physiology·2013

関連する実験動画

Updated: Jul 16, 2026

Iterative Optimization of DNA Duplexes for Crystallization of SeqA-DNA Complexes
11:42

Iterative Optimization of DNA Duplexes for Crystallization of SeqA-DNA Complexes

Published on: November 1, 2012

dUTPaseの結晶構造について

E S Cedergren-Zeppezauer1, G Larsson, P O Nyman

  • 1Department of Zoological Cell Biology, Wenner-Gren Institute, University of Stockholm, Sweden.

Nature
|February 20, 1992
PubMed
まとめ

エシェリキア・コライのdUTPaseは, uracil がDNAに組み込まれるのを防ぐ. X線結晶学により3D構造が明らかになり,ゼリーロールの折りたたみを持つトリメア酵素と,触媒にとって潜在的に重要な複雑なサブユニット相互作用が示されました.

科学分野:

  • バイオケミストリー バイオケミストリー
  • 構造生物学 構造生物学とは
  • 酵素学 酵素学とは

背景:

  • dUTPase酵素は,dUTPを水解することによって, uracil がDNAに組み込まれるのを防ぐ.
  • Escherichia coliのdUTPaseは,核酸を砂糖と塩基によって区別する厳密な基板特異性を示しています.
  • 低細胞内dUTPレベルを維持することは,DNAの完全性にとって非常に重要です.

研究 の 目的:

  • Escherichia coli dUTPaseの3次元構造を決定するために.
  • dUTPaseの基板特異性に対する構造的基礎を解明する.
  • 構造的特徴に基づく潜在的触媒メカニズムを調査する.

主な方法:

  • 酵素の構造を決定するために,X線結晶学を用いた.
  • 高解像度データ収集は1.9年.
  • 三次と四次構造の分析.

主要な成果:

  • E. coli dUTPaseの3次元構造が解明されました.
  • 酵素は対称的なトリマーを形成する.
  • 三次構造は,ジェリーロールの折りたたみで,古典的な核酸結合ドメインとは異なる.

さらに関連する動画

Nucleoside Triphosphates - From Synthesis to Biochemical Characterization
15:22

Nucleoside Triphosphates - From Synthesis to Biochemical Characterization

Published on: April 3, 2014

Combining Non-reducing SDS-PAGE Analysis and Chemical Crosslinking to Detect Multimeric Complexes Stabilized by Disulfide Linkages in Mammalian Cells in Culture
09:37

Combining Non-reducing SDS-PAGE Analysis and Chemical Crosslinking to Detect Multimeric Complexes Stabilized by Disulfide Linkages in Mammalian Cells in Culture

Published on: May 2, 2019

関連する実験動画

Last Updated: Jul 16, 2026

Iterative Optimization of DNA Duplexes for Crystallization of SeqA-DNA Complexes
11:42

Iterative Optimization of DNA Duplexes for Crystallization of SeqA-DNA Complexes

Published on: November 1, 2012

Nucleoside Triphosphates - From Synthesis to Biochemical Characterization
15:22

Nucleoside Triphosphates - From Synthesis to Biochemical Characterization

Published on: April 3, 2014

Combining Non-reducing SDS-PAGE Analysis and Chemical Crosslinking to Detect Multimeric Complexes Stabilized by Disulfide Linkages in Mammalian Cells in Culture
09:37

Combining Non-reducing SDS-PAGE Analysis and Chemical Crosslinking to Detect Multimeric Complexes Stabilized by Disulfide Linkages in Mammalian Cells in Culture

Published on: May 2, 2019

  • 複合的なサブユニット相互作用が四次構造の中で観察されました.
  • 保存された配列要素は,これらの相互作用の近くに位置しています.
  • 結論:

    • 決定された構造は,E. coli dUTPase.の分子構造についての洞察を提供します.
    • サブユニット相互作用を含むユニークな構造的特徴は,触媒作用において役割を果たす可能性があります.
    • さらなる研究によって,これらの構造的発見の機能的意味を調査することができます.