人間のオキシトシン受容体の構造と発現
1Department of Obstetrics and Gynecology, Osaka University Medical School, Japan.
Nature
|April 9, 1992
まとめ
研究者は,ヒトのオキシトシン受容体の構造と発現を特定しました. ミオメトリウム内のオキシトシン受容体メッセンジャーRNAの増加は,出産中の感受性の高まりに寄与する.
科学分野:
- 生殖生物学 生殖生物学
- 分子内分泌学は分子内分泌学である.
- Gタンパク質に結合した受容体.
背景:
- 子宮筋膜のオキシトシンに対する感受性は,出産前に劇的に増加します.
- この高い感受性は,オキシトシン受容体の有意な増加と関連しています.
- オキシトシン受容体の構造と発現を理解することは,出産の始まりを理解するために重要です.
研究 の 目的:
- 人間のオキシトシン受容体補完DNA (cDNA) の構造と発現を決定する.
- オキシトシン受容体の機能的反応を調査する.
- 労働中に増えたミオメトリアオキシトシン受容体のレベルを基礎とする分子メカニズムを解明する.
主な方法:
- 表現クローニングは,ヒトオキシトシン受容体cDNAを分離するために使用されました.
- 受容体の構造を分析し,典型的なGタンパク質結合受容体特性を明らかにした.
- オキシトシン反応を評価するために,Xenopusの卵細胞の受容体発現を伴う機能研究.
- マッセンジャーRNA (mRNA) のサイズとレベルは,様々なヒト組織で分析されました.
主要な成果:
- 人間のオキシトシン受容体は388アミノ酸ポリペプチドで,7つのトランスメブランドメインを持っています.
- クセノプス・オオサイトンの発現したオキシトシン受容体は,オキシトシンに特異的に反応し,膜電流を誘発した.
- オキシトシン受容体mRNAの2つのサイズ (3.6kbおよび4.4kb) が異なる組織で検出されました.
- 4. 4kbのmRNAの非常に高いレベルが,出産後の子宮内膜で観察されました.
結論:
- 特定されたヒトオキシトシン受容体構造は,Gタンパク質結合受容体と一致しています.
- 機能的表現は,オキシトシンの受容体経由による特定の作用を確認します.
- 出産時に観察される受容体数の増加には,出産時にミオメトリアオキシトシン受容体mRNAの大幅な増加が役割を果たしています.
さらに関連する動画
08:37Identification of Orexin and Endocannabinoid Receptors in Adult Zebrafish Using Immunoperoxidase and Immunofluorescence Methods
Published on: June 25, 2019
05:13Estimation of Structural Sensitivity of Intrinsically Disordered Regions in Response to Hyperosmotic Stress in Living Cells Using FRET
Published on: January 12, 2024
関連する概念動画
Enzyme-linked Receptors
Enzyme-linked receptors are proteins that act as both receptor and enzyme, activating multiple intracellular signals. This is a large group of receptors that include the receptor tyrosine kinase (RTK) family. Many growth factors and hormones bind to and activate the RTKs.
Neurotrophin (NT) receptors are a family of RTKs, including trkA, trkB, and trkC (tropomyosin-related kinase) receptors. TrkA is specific for nerve growth factor (NGF), neurotrophin-6, and neurotrophin-7. TrkB binds...
Neurotrophin (NT) receptors are a family of RTKs, including trkA, trkB, and trkC (tropomyosin-related kinase) receptors. TrkA is specific for nerve growth factor (NGF), neurotrophin-6, and neurotrophin-7. TrkB binds...
Intracellular Hormone Receptors
Lipid-soluble hormones diffuse across the plasma and nuclear membrane of target cells to bind to their specific intracellular receptors. These receptors act as transcription factors that regulate gene expression and protein synthesis in the target cell
Enzyme-linked Receptors
Enzyme-linked receptors are proteins that act as both receptor and enzyme, activating multiple intracellular signals. This is a large group of receptors that include the receptor tyrosine kinase (RTK) family. Many growth factors and hormones bind to and activate the RTKs.
Neurotrophin (NT) receptors are a family of RTKs, including trkA, trkB, and trkC (tropomyosin-related kinase) receptors. TrkA is specific for nerve growth factor (NGF), neurotrophin-6, and neurotrophin-7. TrkB binds...
Neurotrophin (NT) receptors are a family of RTKs, including trkA, trkB, and trkC (tropomyosin-related kinase) receptors. TrkA is specific for nerve growth factor (NGF), neurotrophin-6, and neurotrophin-7. TrkB binds...
Transducer Mechanism: G Protein–Coupled Receptors
G Protein–Coupled Receptors (GPCRs) are membrane-bound receptors that transiently associate with heterotrimeric G proteins and induce an appropriate response to various stimuli. GPCRs regulate critical physiological pathways and are excellent drug targets for treating diseases such as diabetes, cancer, obesity, depression, or Alzheimer's. Nearly 35% of approved drugs implement their therapeutic effects by selectively interacting with specific GPCRs.
GPCRs are also called heptahelical, 7TM, or...
GPCRs are also called heptahelical, 7TM, or...
Transducer Mechanism: Enzyme-Linked Receptors
Enzyme-linked receptors are cell-surface receptors acting as an enzyme or associating with an enzyme intracellularly. They make excellent drug targets. Drugs can bind to the extracellular ligand-binding domain or directly affect their enzymatic domain and alter their activity.
Major types that are helpful drug targets include:
Major types that are helpful drug targets include:
Olfactory Receptors: Location and Structure
The process of olfaction, also known as the sense of smell, is a sophisticated chemical response system. The specialized sensory neurons that facilitate this process, known as olfactory receptor neurons, are situated in an upper segment of the nasal cavity, known as the olfactory epithelium. Olfactory sensory neurons are bipolar, with their dendrites extending from the epithelium's apex into the mucus that lines the nasal cavity. Airborne molecules, when inhaled, traverse the olfactory...
