アルファおよびベータT細胞受容体アレルの排除および含有

P Borgulya¹, H Kishi, Y Uematsu

¹Basel Institute for Immunology, Switzerland.

Cell

|May 1, 1992

まとめ

T細胞受容体 (TCR) 遺伝子の再編成は,表面発現後の未成熟のチモサイトでも継続されます. アルファロカスでの再結合は,胸腺における陽性選択後にのみ停止する.

科学分野:

免疫学免疫学とは
分子生物学は分子生物学である.
発達生物学発達生物学とは

背景:

T細胞受容体 (TCR) 遺伝子の再編成は,適応免疫にとって極めて重要です.
T細胞発達の過程におけるTCR遺伝子再配列の正確なタイミングと調節は完全に理解されていません.

研究の目的:

T細胞発達の過程におけるTCR遺伝子再配列の調節を調査する.
ティモサイトの成熟と選択に関連して,TCR遺伝子の再配列が停止する時期を決定する.

主な方法:

アルファベータTCRトランスジェニックマウスにおけるTCR遺伝子発現と再配列の分析.
発達の様々な段階にあるチモサイトの単細胞分析.
RAG-1およびRAG-2の遺伝子発現の定量化.

主要な成果:

トランスジェニックTCRは未成熟のチモサイトで早期に発現する.
未成熟のチモサイトは,トランスジェニックのTCRと共に内生性アルファTCR鎖を発現することができる.
RAG-1とRAG-2の遺伝子発現は,成熟したチモサイトのポジティブな選択後の発現に減少する.

結論:

アルファベータTCRの表面発現は,さらなるTCRアルファ遺伝子の再配列を直ちに停止しません.
TCR遺伝子の再結合は,特にアルファロカスでは,胸腺における陽性選択の後まで続きます.
発達中のT細胞は,胸膜の選択中にMHCリガンド結合のための複数のTCRを探索することがあります.

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