T細胞抗原受容体の活性化誘発によるユビキチン化

C Cenciarelli¹, D Hou, K C Hsu

¹Experimental Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892.

Science (New York, N.Y.)

|August 7, 1992

まとめ

T細胞抗原受容体 (TCR) のゼータサブユニットは,活性化時にユビキチン化されます. この翻訳後の改変は,超膜受容体シグナル伝達におけるユビキチネーションのより広範な役割を示唆しています.

科学分野:

免疫学免疫学とは
分子生物学は分子生物学である.
細胞シグナル伝達細胞信号伝達

背景:

ゼータサブユニットは,T細胞受容体 (TCR) 媒介の信号伝達に不可欠です.
ディスルファイド結合ホモジマーとして機能し,TCR活性化タンパク質チロシンキナーゼの基質である.

研究の目的:

レセプターエンゲージメント時のTCRゼータ鎖の翻訳後の修正を調査する.
UbiquitinationがTCRシグナル伝達に作用するかどうかを判断する.

主な方法:

TCR活性化後のT細胞と腫瘍線の分析.
生化学的測定を用いたTCRサブユニットのユビキチン化検出.

主要な成果:

TCRゼータ鎖は,正常なT細胞と腫瘍系の両方で受容体の関与に反応して,ユビキチン化を経験します.
ゼータの酸化と非酸化の両方の形態は,どこにでも存在していた.
また,TCRの別の成分であるCD3デルタサブユニットも,ユビキチン化されていることが判明しました.

結論:

ユビキチネーションは,活性化時にTCRゼータ鎖とCD3デルタサブユニットの重要な翻訳後の改変である.
これらの発見は,超膜受容体の機能,特に免疫信号伝達経路におけるユビキチネーションの役割の拡大を示しています.

さらに関連する動画

07:48

Spatial and Temporal Control of T Cell Activation Using a Photoactivatable Agonist

Published on: April 25, 2018

08:33

Isolation, Cultivation, and Transient Transfection of Primary Human T Cells to Generate Chimeric Antigen Receptor (CAR) T Cells

Published on: March 27, 2026

関連する概念動画

01:28

Diversity of Antigen Receptors

Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...

01:22

Antigen Presenting Cells

The immune system is a complex network of cells and molecules that protects the body from foreign invaders. T cells, a type of white blood cell, play a crucial role in this process. They recognize and attack foreign substances, such as pathogens, that enter the body.
T cells require the help of antigen-presenting cells (APCs), which process foreign antigens into smaller fragments that can be recognized by T cells. These APCs are highly specialized cells that efficiently internalize antigens...

01:22

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...

01:24

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...

01:27

Cytotoxic T Cells-mediated Immune Response

Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...

01:24

B Cell Activation and Differentiation

The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...

関連する実験動画