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Updated: Jul 17, 2026

Identification of Protein Complexes in Escherichia coli using Sequential Peptide Affinity Purification in Combination with Tandem Mass Spectrometry
14:58

Identification of Protein Complexes in Escherichia coli using Sequential Peptide Affinity Purification in Combination with Tandem Mass Spectrometry

Published on: November 12, 2012

E. coliにおけるDNAとタンパク質の相互作用を in vivo で全ゲノムアプローチで調べる

M X Wang1, G M Church

  • 1Laboratory of Oncology Research, Wills Eye Hospital, Philadelphia, Pennsylvania.

Nature
|December 10, 1992
PubMed
まとめ

この研究では,タンパク質によって保護されたDNA配列を in vivo で識別するための全ゲノム法が導入されています. この技術は,メチラゼとメチレーションに敏感な酵素を使用して,これらの保護された部位をマッピングし,遺伝子調節にとって重要なタンパク質-DNA相互作用を明らかにします.

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