関連する実験動画
Updated: May 10, 2026

09:04
Detection of Neu1 Sialidase Activity in Regulating TOLL-like Receptor Activation
Published on: September 8, 2010
ニュー分化因子:EGFドメインと免疫グロブリンホモロジー単位を含むトランスメブラングリコタンパク質
1Department of Chemical Immunology, Weizmann Institute of Science, Rehovot, Israel.
Cell
|May 1, 1992
まとめ
新種のタンパク質であるノイ分化因子 (NDF) は,乳がん腫瘍細胞の分化と成長停止を刺激する. NDFは,ノイ・プロト・オンコゲン・プロダクトを活性化し,細胞の行動に影響を与えるグリコタンパク質です.
科学分野:
- 分子生物学は分子生物学である.
- 細胞生物学 細胞生物学
- 腫瘍学 腫瘍学
背景:
- 変異した線維芽細胞は44kDのグリコタンパク質を分泌する.
- このタンパク質は,新原発がん原産物のチロシンリン酸化を刺激する.
- また,乳がん腫瘍細胞の分化も誘導する.
研究 の 目的:
- ノイ分化因子 (NDF) の補完DNAをクローンする.
- NDFの構造と機能を明らかにする.
- NDF表現の規制を調査する.
主な方法:
- NDFの部分的アミノ酸配列.
- 補完的なDNAクローン.
- COS-7細胞における再結合タンパク質発現.
- ノーザン・ブロット分析.
主要な成果:
- NDF前駆体は,EGF型と免疫グロブリンホモロジーのドメインを持つトランスメブランタンパク質です.
- 再結合NDFは生物学的に活性であり,分泌されます.
- NDF発現は,ras oncogene-transformed fibroblastsにおいて上昇している.
- 複数のNDFトランスクリプト (1.8,2.6,6.7kb) が存在する.
結論:
- NDFは乳がん腫瘍細胞の分化と成長停止に作用する.
- NDFの構造は,受容体-リガンドの相互作用を示唆しています.
- Rasの腫瘍遺伝子の変換は,NDF発現を上調する.
関連する概念動画
Enzyme-linked Receptors
Enzyme-linked receptors are proteins that act as both receptor and enzyme, activating multiple intracellular signals. This is a large group of receptors that include the receptor tyrosine kinase (RTK) family. Many growth factors and hormones bind to and activate the RTKs.
Neurotrophin (NT) receptors are a family of RTKs, including trkA, trkB, and trkC (tropomyosin-related kinase) receptors. TrkA is specific for nerve growth factor (NGF), neurotrophin-6, and neurotrophin-7. TrkB binds...
Neurotrophin (NT) receptors are a family of RTKs, including trkA, trkB, and trkC (tropomyosin-related kinase) receptors. TrkA is specific for nerve growth factor (NGF), neurotrophin-6, and neurotrophin-7. TrkB binds...
Neurulation
Neurulation is the embryological process which forms the precursors of the central nervous system and occurs after gastrulation has established the three primary cell layers of the embryo: ectoderm, mesoderm, and endoderm. In humans, the majority of this system is formed via primary neurulation, in which the central portion of the ectoderm—originally appearing as a flat sheet of cells—folds upwards and inwards, sealing off to form a hollow neural tube. As development proceeds, the anterior...
Mitogens and the Cell Cycle
Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
Receptor Downregulation in MVBs
Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR activation may...
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR activation may...
Immunoglobulin-like Cell Adhesion Molecules
Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal
Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.

