アミノペプチダゼNは,腸内病原性コロナウイルスTGEVの主要な受容体です
B Delmas1, J Gelfi, R L'Haridon
1Unité de Virologie et Immunologie Moléculaires, INRA, Jouy-en-josas, France.
Nature
|June 4, 1992
まとめ
伝染性胃腸炎ウイルス (TGEV) は,ブタを感染させるための受容体としてアミノペプチダゼNを使用します. この発見は,TGEVが宿主細胞に侵入するための重要な分子決定因子を特定しています.
科学分野:
- 獣医ウイルス学 獣医ウイルス学
- 分子生物学は分子生物学である.
- 細胞生物学 細胞生物学
背景:
- コロナウイルスは限られた宿主範囲と組織トロピズムを示します.
- 伝染性胃腸炎ウイルス (TGEV) は,小腸腸内細胞を感染させ,仔豚に致命的な下痢を引き起こす.
研究 の 目的:
- TGEVの結合と侵入を媒介する特定の表面分子を特定するために.
- TGEV宿主細胞の相互作用の分子メカニズムを解明する.
主な方法:
- 豚の細胞系におけるTGEV感染を阻害するモノクローナル抗体の選択.
- 抗体認識とアミノ端末配列決定を用いた標的タンパク質の識別.
- 標的タンパク質の浄化とウイルス結合の評価.
- 特定されたタンパク質の再結合表現は,非許容性細胞で.
主要な成果:
- 150Kのブラッシュ・ボーダー膜タンパク質は,アミノペプチダゼNとして識別されました.
- アミノペプチダゼNがTGEV受容体であることを,特定のバイリオン結合アッセイで確認した.
- 再結合アミノペプチダースのN発現は,TGEV感染に敏感な非許容性細胞を表現した.
結論:
- アミノペプチダゼNは,豚の腸内細胞における伝染性胃腸炎ウイルス (TGEV) の主要な受容体である.
- この相互作用を理解することは,TGEV制御戦略の開発に不可欠です.
- この研究は,コロナウイルスの病原性における重要なウイルス-宿主決定因子を明らかにしています.
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