長期の血液形成培養でヒトの帯血の増殖
J M Hows1, B A Bradley, J C Marsh
1Kay Kendall Laboratory, Paterson Institute, Cancer Research Campaign, Manchester, UK.
Lancet (London, England)
|July 11, 1992
まとめ
人間の帯 (HUC) 血液は,移植のための骨髄と比較して,優れた血液生成性幹細胞を提供しています. HUC血液は,よりよい成長の可能性と細胞量を示しており,成人では単発の献血が十分である可能性を示唆しています.
科学分野:
- 血液学 ヘマトロジ
- 幹細胞生物学 幹細胞生物学
- 移植医学 移植医学について
背景:
- 凍結保存されたヒト帯 (HUC) 血液は,血液生成性幹細胞移植のための骨髄の新たな代替手段です.
- HUC血液は,HLA同型移植を必要とする白血病またはファンコニ貧血の小児患者にとって特に重要です.
研究 の 目的:
- HUC血液幹細胞のインビトロ成長可能性を評価するために.
- HUC血液から得られた血液形成性幹細胞の量と質を,成人骨髄と比較するために.
主な方法:
- コロニー形成能力を評価するためにクローノジェニックアッセイを用いた.
- 放射線を浴びた骨髄ストロマに長期にわたる造血培養を用いて,原始細胞の産生と培養寿命を評価した.
主要な成果:
- HUC血液は,クローノジェニックアッセイで成人骨髄と同等のコロニーの数値を示し,原始細胞コロニーの比率が高かった.
- 長期培養では,骨髄と比較してHUC血による原始細胞の生産と培養寿命が著しく増加した (p = 0.0007).
結論:
- HUC血系血型幹細胞は,正常な成人の骨髄と比較して,質と量が優れている.
- 成人の移植には1回のHUC献血が十分で,銀行やより広範な臨床応用への考慮を支持する可能性がある.
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