精子生成中のCREM機能の発達的な切り替え:アンタゴニストからアクティベーターへ
N S Foulkes1, B Mellström, E Benusiglio
1Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, Strasbourg, France.
Nature
|January 2, 1992
まとめ
研究者らは,CREM tauと呼ばれるCREM遺伝子の新しい形態を成人の丸で発見しました. このアイソフォームは,精子発達の過程で抑制から活性化に切り替え,これは男性の生殖能力にとって極めて重要です.
科学分野:
- 生殖生物学 生殖生物学
- 分子遺伝学 分子遺伝学
- 細胞シグナリング
背景:
- 精子生成は, pituitary-hypothalamic axisとアデニル酸サイクラゼシグナル伝達によって調節される.
- CREM遺伝子は,cAMPの転写応答を調節する代替スプライシングの変種を生成する.
研究 の 目的:
- 成人哺乳類の丸における新しいCREM同型を特定し,特徴づけること.
- 精子生成中のCREMイソフォームの機能的役割を調査する.
主な方法:
- 精巣細胞における遺伝子発現とスプライシングパターンの分析.
- 新型CREMイソフォームのタンパク質ドメインと転写活性の特徴化.
主要な成果:
- 新型CREM同型,CREMタウは,成人の丸で特定されました.
- CREMタウはグルタミンに富んだ2つのドメインを有し,転写活性化を授与する.
- CREMの発現は,プレミオティック細胞における抗体形態からアクティベーター形態 (CREM tau) にパキテン段階から切り替わります.
結論:
- CREMタウの発見は,精子生成中の遺伝子発現の調節におけるCREMタウの重要な役割を強調しています.
- CREMのスプライシング依存機能的逆転は,男性生殖細胞の発達中の遺伝子発現の発達変調のための重要なメカニズムです.
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