L-セレクチンの内皮リガンドは,新しいムシンのような分子である
L A Lasky1, M S Singer, D Dowbenko
1Department of Immunobiology, Genentech, Inc,, South San Francisco, California 94080.
Cell
|June 12, 1992
まとめ
研究者らは,リンパ節に新しいムシンのようなグリコプロテインを特定し,それは支架として機能します. この分子は,L-セレクチンに炭水化物を提示し,免疫監視のために,リンパ球の粘着を高い内皮静脈 (HEV) に媒介する.
科学分野:
- 免疫学 免疫学とは
- 細胞生物学 細胞生物学
- 分子生物学は分子生物学である.
背景:
- リンパ球がリンパ節にたどり着くことは,免疫反応にとって極めて重要です.
- L-セレクチンは,リンパ球の粘着を高内皮静脈 (HEV) に媒介する.
- 硫酸グリコタンパク質 (Sgp50) は,L-セレクチンに対するHEVリガンドとして以前に特定されました.
研究 の 目的:
- Sgp50グリコタンパク質を浄化する.
- Sgp50.0.をコードするcDNAをクローンするために.
- リンパ球-HEV相互作用におけるSgp50の構造と機能を特徴付ける.
主な方法:
- Sgp50グリコプロテインの浄化.
- N端のアミノ酸の配列.
- cDNAクローニングと配列分析.
- mRNA発現分析. mRNA発現分析. mRNA発現分析. mRNA発現分析. mRNA発現分析. mRNA発現分析. mRNA発現分析. mRNA発現分析. mRNA発現分析.
- 抗体生成と免疫降水.
- リンパ節HEVsの免疫ヒストロケミカル染色.
主要な成果:
- Sgp50の浄化とcDNAクローニングは成功しました.
- 予測されたタンパク質配列は,セリン/スレオニンに富んだドメインを持つ新しいムシンのような分子を明らかにした.
- Sgp50 mRNAはリンパ節で好ましく発現する.
- Sgp50に対する抗体は,グリコプロテインを免疫し,HEV表面を染色した.
- Sgp50は,炭水化物をL-セレクチンに提示する支架として機能します.
結論:
- 新しい組織特異的なムシンのような内皮糖タンパク質 (Sgp50) が特定されました.
- このグリコタンパク質は,リンパ球がリンパ節HEVに接着するのを媒介する上で重要な役割を果たします.
- Sgp50は,L-セレクチンレクチン領域に炭水化物リガンドを提示することによって機能する可能性があります.
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