T細胞ハイブリドトーマにおける活性化誘発によるアポプトシス細胞死におけるc-mycの役割

Y Shi¹, J M Glynn, L J Guilbert

¹Department of Immunology, University of Alberta, Edmonton, Canada.

Science (New York, N.Y.)

|July 10, 1992

まとめ

T細胞の活性化誘発アポトシスには,c-myc遺伝子発現が必要です. c-mycをブロックすると,活性化後のT細胞死が防止され,免疫細胞の調節における重要な役割を強調します.

科学分野:

免疫学免疫学とは
分子生物学は分子生物学である.
細胞生物学細胞生物学

背景:

未成熟のT細胞とT細胞ハイブリドオマは,T細胞受容体活性化時にアポトーシスを受けます.
このプロセスは,発達中のT細胞の負の選択に関与している可能性が高い.
アクティベーション誘発アポトーシスは,活性タンパク質とRNA合成を必要としますが,特定の遺伝子は特定されていません.

研究の目的:

T細胞における活性化誘発アポトーシスに不可欠な遺伝子を特定する.
活性化後のT細胞アポトーシスにおけるc-mycの役割を調査する.

主な方法:

c-myc.を標的とした反意味オリゴヌクレオチドを使用した.
T細胞受容体複合体を通して活性化された研究されたT細胞ハイブリドトーマ.
アポプトーシスとリンフォキンの産生に対する評価された効果.

主要な成果:

c-Mycに対するアンチセンセスのオリゴヌクレオチドは,構成的なc-Mycタンパク質発現を成功裏に阻害しました.
c-myc発現への干渉は,活性化誘発のアポトーシスのすべての側面を抑制しました.
リンフォキンの生成はc-myc阻害によって影響を受けませんでした.

結論:

c-myc遺伝子発現は,T細胞の活性化誘発性アポトーシスに必要な要素である.
c-mycは,活性化後のT細胞生存を調節する上で重要な役割を果たします.
c-mycを標的にすることは,T細胞アポトーシスを調節するための戦略かもしれません.

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