Annexxin VIは,クラトリンでコーティングされた穴の芽生えのために必要です

H C Lin¹, T C Südhof, R G Anderson

¹Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas 75235-9039.

Cell

|July 24, 1992

まとめ

アネキシンVIは,プラズマ膜からクラトリンで覆われた穴の芽生えに不可欠です. サイトゾール,ATP,およびカルシウムイオンは,附属書VIに必要なものです.

科学分野:

細胞生物学細胞生物学
メンブラン密輸膜密輸
タンパク質の生化学

背景:

クラトリンで覆われた穴は,内分細胞形成に不可欠です.
プラズマ膜からの脱離には,特定の細胞因子が必要です.

研究の目的:

クラスリンで覆われたピッツの芽生えにおけるAnnex VIの役割を調査する.
このプロセスを調節するシトソール内の要因を特定する.

主な方法:

隔離されたプラズマ膜を使用した.
サイトゾールは,附属書VIから取り除かれ,再構成された.
芽生え活動を測定したのは,精製されたアネキシンVI,ATP,Ca2+が含まれており,含まれておらずでした.

主要な成果:

サイトゾールからのAnnexin VIの枯渇は,コーティングされたピットブーディングを廃止しました.
浄化された附属書VIの再添加が芽を咲かせた.
完全な活動には,追加の細胞分裂因子,ATP,およびCa2+が必要でした.

結論:

アネキシンVIは,クラスリンで覆われた膀形成の重要な成分です.
サイトゾールには,アネキシンVIの活性を調節する因子が含まれており,ATPとCa2+に依存しています.
アネキシンVIは,コーティングされた膀の形成の分離と調節の両方に役割を果たします.

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