インターフェロンアルファ/ベータシグナル伝達経路におけるタンパク質チロシンキナーゼ
L Velazquez1, M Fellous, G R Stark
1Unité INSERM 276, Institut Pasteur, Paris, France.
Cell
|July 24, 1992
まとめ
研究者らは,タキ2遺伝子を特定し,インターフェロンαに反応しないヒト細胞系を修正した. この発見は, tyk2 を明らかにしています.
科学分野:
- 分子生物学は分子生物学である.
- 免疫学 免疫学とは
- 遺伝学 遺伝学とは
背景:
- 変異したヒト細胞系11.1は,インターフェロンアルファに反応しない.
- この無反応を引き起こす特定の遺伝的欠陥は,以前は知られていなかった.
研究 の 目的:
- 変異細胞系を遺伝的に補完する 11.1.1.
- インターフェロンアルファ反応の回復を担う野生型の遺伝子を特定し,クローン化します.
主な方法:
- 変異細胞をゲノムDNAに感染させる.
- 強力なバックセレクション戦略を用いて,修正DNAを分離した.
- コズミッド分離,補完的なDNA (cDNA) クローン,および配列分析.
主要な成果:
- ミュータントのフェノタイプを逆転させたコスミッドが分離されました.
- コスミッドには,突然変異した細胞に大きく縮小された単一のメッセージが含まれていた.
- クローンされた遺伝子は,ヒトの非受容体タンパク質チロシンキナーゼであるTyk2として識別されました.
結論:
- tyk2遺伝子は,インターフェロンアルファシグナル伝達に不可欠です.
- tyk2は,インターフェロンα/β受容体と下流転写因子の間の重要なリンクとして作用します.
- これは,インターフェロン反応性遺伝子活性化を媒介する tyk2 の機能を特定します.
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