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翻訳機構と70kdの熱ショックタンパク質は,タンパク質合成に協力する
R J Nelson1, T Ziegelhoffer, C Nicolet
1Department of Biomolecular Chemistry, University of Wisconsin-Madison 53706.
Cell
|October 2, 1992
まとめ
酵母熱ショックタンパク質 (Hsp70s) は,リボソームと新生ポリペプチドと結合し,タンパク質合成を支援します. これらのタンパク質が欠けている変異株は,成長の遅さとタンパク質合成の欠陥を示す.
科学分野:
- 分子生物学は分子生物学である.
- 細胞生物学 細胞生物学
- タンパク質の折りたたみ
背景:
- 熱ショックタンパク質 (Hsp70s) は,タンパク質ホメオスタシスに関与する重要な分子チャペロンである.
- サイトソリックHsp70s,特に酵母中のSSBタンパク質 (Ssb1/2p) は,タンパク質合成に関与しています.
- 翻訳プロセスにおけるSSB Hsp70sの正確な役割は,まだ完全に理解されていません.
研究 の 目的:
- 酵母SSB 70 kd熱ショックタンパク質 (hsp70s) のタンパク質合成における機能を明らかにする.
- SSB Hsp70sの翻訳リボソームと新生ポリペプチドとの相互作用を調査する.
- SSB Hsp70機能障害の生理学的結果を理解するために.
主な方法:
- バイオケミカルアッセイにより,Ssb1/2pとリボソームの翻訳との関連を決定する.
- SSB遺伝子 (ssb1 ssb2) に変異がある酵母菌株の分析.
- 変異株のフェノタイプ的特徴,成長率とタンパク質合成阻害剤に対する感受性など.
- 翻訳延伸因子1α (EF-1α) のような遺伝子の過剰発現を含む遺伝子解析.
主要な成果:
- SSB Hsp70s (Ssb1/2p) はリボソームの翻訳と関連していることが判明しました.
- ピューロマイシン治療は,その関連性を破壊し,新生ポリペプチドとの結合を示唆した.
- 変異したssb1 ssb2株は,成長が遅い,リボソームの翻訳が低下し,タンパク質合成阻害剤に対する過敏性を示した.
- 成長が遅いフェノタイプは,新しいEF-1α型タンパク質の発現の増加によって抑制されました.
結論:
- サイトソリックSSB Hsp70sは,新生ポリペプチドがリボソームを通過するのを支援する上で重要な役割を果たします.
- この機能は,タンパク質が膜を横断する輸送に関与するオルゲネル局所化されたHsp70sに類似しています.
- SSB Hsp70sは,酵母における効率的なタンパク質合成と細胞成長に不可欠です.
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