MycとMaxのタンパク質は,異なった転写活動を持っています
L Kretzner1, E M Blackwood, R N Eisenman
1Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.
Nature
|October 1, 1992
まとめ
Mycタンパク質は転写を活性化し,Maxタンパク質はそれを抑制する. MycとMaxの共同発現は,この抑圧を緩和し,遺伝子調節における複雑な役割を強調します.
科学分野:
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
- 細胞生物学 細胞生物学
背景:
- Mycファミリーのタンパク質は,基本ヘリックス・ループ・ヘリックス・ジッパー (bHLH-Z) ドメインを有しており,転写因子の活性を示唆しています.
- Mycタンパク質は,bHLH-Zタンパク質Maxと異体化し,特定のDNA結合複合体を形成する.
研究 の 目的:
- 哺乳類の細胞におけるMycおよびMaxタンパク質の転写活動を調査する.
- レポーター遺伝子解析を通して,遺伝子調節におけるマイクとマックスの役割を解明する.
主な方法:
- 哺乳類の細胞におけるMycおよびMaxタンパク質の過剰発現.
- 転写活性を測定するためにレポーター遺伝子解析を用いた.
- タンパク質ドメインが転写調節に及ぼす影響を分析する.
主要な成果:
- Mycの過剰発現は,レポーター遺伝子転写を活性化させた.
- Maxの過剰発現は,レポーター遺伝子転写を抑制しました.
- c-Mycの共同発現は,Myc-Maxの結合部位に依存するMax誘発抑制を緩和しました.
結論:
- Mycタンパク質は,転写活性化剤として機能する.
- マックスタンパク質は,転写抑制剤として作用する.
- MycとMaxの相互作用は,特定のDNA結合とタンパク質とタンパク質の相互作用を含む遺伝子転写の調節に不可欠です.
関連する概念動画
02:41Chromatin Structure Regulates pre-mRNA Processing
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
The chromatin structure, especially...
02:04Co-activators and Co-repressors
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
02:42Eukaryotic Transcription Activators
Transcription activators are proteins that promote the transcription of genes from DNA to RNA. In most cases, these proteins contain two separate domains ‒ a domain that binds to DNA and a domain for activating transcription; however, in some cases, a single domain is responsible for both binding and activation of transcription, as seen in the glucocorticoid receptor and MyoD.
The binding domains are capable of recognizing and interacting with regulatory sequences on the DNA. These domains are...
The binding domains are capable of recognizing and interacting with regulatory sequences on the DNA. These domains are...
02:23Master Transcription Regulators
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
02:04Co-activators and Co-repressors
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
02:23Master Transcription Regulators
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...