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関連する概念動画

Overview of Electron Microscopy01:25

Overview of Electron Microscopy

The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
Transmission Electron Microscopy01:15

Transmission Electron Microscopy

In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an optical microscope—developed the first prototype of the electron microscope. This development led to the development of the field of electron microscopy. In the transmission electron microscope (TEM), electrons are produced by a hot tungsten element and accelerated by a potential difference in an electron gun, which gives them up to 400 keV in...
Cryo-electron Microscopy01:28

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...

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関連する実験動画

Updated: Jul 9, 2026

Multimodal Hierarchical Imaging of Serial Sections for Finding Specific Cellular Targets within Large Volumes
11:19

Multimodal Hierarchical Imaging of Serial Sections for Finding Specific Cellular Targets within Large Volumes

Published on: March 20, 2018

分子から細胞へ:原子力顕微鏡で柔らかいサンプルを画像化

M Radmacher1, R W Tillamnn, M Fritz

  • 1Physikdepartment, Technische Universität München, 8046 Garching, Germany.

Science (New York, N.Y.)
|September 25, 1992
PubMed
まとめ
この要約は機械生成です。

原子力顕微鏡 (AFM) の画像は,硬質のサンプルをよく撮りますが,軟質の有機サンプルには依然として課題があります. 新しいAFM方法により,現在,高解像度画像と,生体細胞のような柔らかい物質の微機械的特性マッピングが可能になっています.

さらに関連する動画

Miniaturized Sample Preparation for Transmission Electron Microscopy
09:04

Miniaturized Sample Preparation for Transmission Electron Microscopy

Published on: July 27, 2018

Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy
09:21

Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy

Published on: July 20, 2019

関連する実験動画

Last Updated: Jul 9, 2026

Multimodal Hierarchical Imaging of Serial Sections for Finding Specific Cellular Targets within Large Volumes
11:19

Multimodal Hierarchical Imaging of Serial Sections for Finding Specific Cellular Targets within Large Volumes

Published on: March 20, 2018

Miniaturized Sample Preparation for Transmission Electron Microscopy
09:04

Miniaturized Sample Preparation for Transmission Electron Microscopy

Published on: July 27, 2018

Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy
09:21

Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy

Published on: July 20, 2019

科学分野:

  • 材料科学 材料科学とは
  • バイオフィジックス 生物物理学
  • 表面科学とは,地表科学である.

背景:

  • 原子力顕微鏡 (AFM) は,高解像度の表面イメージングのための強力な近地技術です.
  • 硬いサンプルには効果的ですが,AFMで柔らかい生物学的および有機的な材料を画像化することは大きな課題です.
  • ソフトサンプルの性質の解明と特徴づけの限界を克服するために,進歩が必要である.

研究 の 目的:

  • 有機サンプルに対する原子力顕微鏡 (AFM) の応用について概要を述べる.
  • 柔らかい物質のAFMにおける基本的な画像形成メカニズムについて議論する.
  • ローカルマイクロメカニカルプロパティ測定のための新しいAFMイメージングモードを導入する.

主な方法:

  • オーダーされた薄膜と生きている細胞にAFMの適用.
  • 画像形成のためのチップサンプルの相互作用メカニズムについての議論.
  • 粘着弾性および摩擦マッピングのための新しいイメージングモードの導入.

主要な成果:

  • AFMは分子解像度で薄型オーダーフィルムを成功裏に画像化しました.
  • 新しいイメージングモードにより,マイクロメカニカル特性の局所的な測定が可能になります.
  • Langmuir-Blodgett フィルムの粘着弾性および摩擦係数をマッピングしました.

結論:

  • AFMは,フィルムから細胞まで,多様な有機サンプルにますます適用されています.
  • 新しいAFM技術は,柔らかい物質の性質の特徴を高めています.
  • AFMは,有機物質の微機械的行動に関する貴重な洞察を提供します.