ヘモグロビン-AGE: 進行したグリコシライゼーションの循環マーカーである
Z Makita1, H Vlassara, E Rayfield
1Picower Institute for Medical Research, Manhasset, NY 11030.
まとめ
先進型グリコシル化最終産物 (AGEs) は,ヘモグロビンを改変し,Hb-AGEを形成する. このマーカーは糖尿病において増加し,治療によって減少し,糖尿病合併症におけるAGEを追跡する可能性を示している.
科学分野:
- バイオケミストリー バイオケミストリー
- エンドクリノロジー エンドクリノロジー
- ゲロントロジーはゲロントロジーの学科です.
背景:
- アドバンスト・グリコシル化最終産物 (AGEs) は,グルコースから形成され,動脈硬化症や腎臓病などの老化および糖尿病合併症に関与しています.
- ヘモグロビンなどの長寿タンパク質は,AGEによって変化することがあります.
- AGEsの蓄積は,組織損傷と疾患の病原化に寄与する.
研究 の 目的:
- AGEによって改変されたヒトのヘモグロビンの形態を特定し,特徴づけること.
- この改変したヘモグロビンのレベルを正常な個人と糖尿病患者で調べる.
- 糖尿病および年齢関連の合併症に対するAGEの貢献を評価する際に,このマーカーの可能性を評価する.
主な方法:
- 最近開発されたAGE特異抗体を検出に利用した.
- AGE変異による特定のヘモグロビン種,ヘモグロビン-AGE (Hb-AGE) を特定し,定量化しました.
- Hb-AGEレベルを測定した正常な個体と糖尿病患者の高血糖症,およびアミノグアニジンの治療中に変化をモニターした.
主要な成果:
- 新しいAGE-改変ヘモグロビンHb-AGEが特定されました.
- Hb-AGEレベルは,糖尿病患者 (0.75%) で正常な個人 (0.42%) に比べて高いことが判明しました.
- AGE阻害剤アミノグアニジンの治療により,1ヶ月以内にHb-AGE濃度が著しく低下しました.
結論:
- Hb-AGEは,AGEによる長期的な組織改変の測定可能なマーカーとして機能します.
- Hb-AGEレベルは,糖尿病における高血糖症とAGE蓄積の程度を反映しています.
- Hb-AGEの測定は,糖尿病および年齢関連の疾患における高度なグリコシライゼーションの影響を評価するための貴重なツールである可能性があります.
さらに関連する動画
09:29Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications
Published on: May 18, 2017
06:26Analyzing the Permeability of the Blood-Brain Barrier by Microbial Traversal through Microvascular Endothelial Cells
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