インタクトおよびネクロティック結合組織のコラーゲノリチス活性

E R GOLDSTEIN, Y M PATEL, J C HOUCK

Science (New York, N.Y.)

|November 13, 1964

まとめ

限られたトリプシンによって活性化されたラット皮のエキスは,溶解性および不溶解性コラーゲンからヒドロキシプロリンを放出します. このコラーゲノリチス作用はpH5.5に依存し,熱に不安定である.

科学分野:

バイオケミストリーバイオケミストリー
皮膚科皮膚科について
酵素学酵素学とは

背景:

コラーゲンは皮膚の重要な構造タンパク質です.
コラーゲンの分解を理解することは,皮膚の生物学と疾患にとって極めて重要です.
皮膚抽出物における内生的なコラーゲノリチス酵素に関する情報は限られている.

研究の目的:

ネズミの皮の抽出物のコラーゲノリチス作用の可能性を調査する.
この活動に影響を与える条件と阻害物質を特徴付ける.

主な方法:

ネズミの皮膚 (無傷および死傷) からイソトニック塩水抽出物の調製.
トリプシンを使用した抽出物の限られたタンパク質分解活性化.
溶性および不溶性コラーゲンからヒドロキシプロリン放出の測定.
pH,阻害剤 (大豆トリプシン阻害剤,EDTA,重金属塩),熱,および広範なタンパク質分解がコラーゲノリチス活性に及ぼす影響を試験する.

主要な成果:

活性化されたラット皮のエキスは,溶性コラーゲンのヒドロキシプロリンの50%以上を放出します.
これらの抽出物はまた,不溶性コラーゲンを溶解させ,ヒドロキシプロリンを含むペプチドを放出します.
最適なコラーゲノリチス作用は,pH 5.5.5 で観察されました.
この活動は,大豆のトリプシン阻害剤,EDTA,または重金属塩によって抑制されませんでした.
変性ヘモグロビンに対する一般的なプロテオリチス作用は存在しない.
コラーゲノリチス作用は,熱と広範なトリプチス消化によって廃止されました.

結論:

ネズミの皮膚には,限られたタンパク質分解によって活性化できる潜在的コラーゲノリチス要因が含まれています.
これらの因子は,一般的なプロテアゼと異なる特定のコラーゲナゼのような活性を示します.
これらの発見は,皮膚のコラーゲン周回量または再構築におけるこれらの活性化因子の役割を示唆しています.

キーワード:

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