ヒトリンパ球における酸・リンパ塩酸に富んだ粒子は,フィトヘマグルチニンによって誘発される

R HIRSCHHORN, J M KAPLAN, A F GOLDBERG

Science (New York, N.Y.)

|January 1, 1965

まとめ

フィトヘマグルトニン (PHA) はヒトリンパ球を刺激し,細胞分裂を引き起こします. ミトーシス前に酸リンファサゼの活性が増え,ライソソーム状の粒子が現れ,PHA駆動のリンパ球増殖の役割を示唆する.

科学分野:

細胞生物学細胞生物学
免疫学免疫学とは
バイオケミストリーバイオケミストリー

背景:

人間のリンパ球は,重要な免疫細胞です.
フィトヘマグルーチニン (PHA) は,リンパ球の増殖を刺激することが知られている植物性レクチンです.
リンパ球の活性化には,複雑な分子および形態学的変化が伴う.

研究の目的:

フィトヘマグルーチニン刺激によるリンパ球の変容における酸リンファーターゼ活動の役割を調査する.
ミトーシスに関連して酸ホスファターゼの増加の細胞の局所化とタイミングを調べる.

主な方法:

ヒトリンパ球の培養は,in vitroで行われました.
フィトヘマグルーチニンはリンパ球の活性化を刺激するために使用されました.
ヒストケミカル染色は,酸ホスファターゼの活性を検出するために使用されました.
形態学的変化とミトスの進行をモニタリングした.

主要な成果:

フィトヘマグルーチニンの刺激により,ヒトリンパ球における形態学的変異とミトーシスが誘発された.
刺激後48~72時間,ミトーシスの前の,酸ホスファタゼ活性の大幅な増加が観察されました.
ヒストキミカル分析により,細胞質内の多数の酸フォスファターゼ陽性粒子の形成が明らかになった.

結論:

アシドフォスファターゼの活性が増加し,ライソソームのような粒子が存在することは,フィトヘマグルーチニン誘発のリンパ球分裂と関連しています.
PHAによって刺激されるリンパ球の増殖のプロセスにおいて,酸リンファタゼを含む粒子が機能的な役割を果たす可能性があります.

キーワード:

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