人間のゲノムの第2世代のリンクマップ

J Weissenbach¹, G Gyapay, C Dib

¹Genethon, Evry, France.

Nature

|October 29, 1992

まとめ

研究者は814の新しい多形マーカーを使用して,ヒトゲノムリンクマップを作成しました. この地図は,ゲノムの90%をカバーし,ほとんどのマーカーを遺伝子研究のために23の染色体グループに分けています.

科学分野:

ゲノミクスゲノミクスとは
人間の遺伝学人間の遺伝学
分子生物学は分子生物学である.

背景:

総合的なヒトゲノムリンクマップを確立することは,遺伝的多様性と病気を理解するために不可欠です.
以前の地図には,マーカー密度とゲノムカバーに関する制限がありました.

研究の目的:

ヒトゲノムの高解像度のリンクマップを構築する.
ゲノム全体に相当数のポリモルフマーカーを特定し,並べ替える.

主な方法:

814の新しい (C-A) n 繰り返しポリモルフのロシエの分離分析.
リンク分析のために8つの大家族からのDNAを使用した.
マーカーの順序とリンクグループを決定するための統計分析.

主要な成果:

814のマーカーのうち813を23の結合群 (22オートソーム+X染色体) にマッピングしました.
605のマーカーで高いヘテロジゴシティ (>0.7) が観察されました.
553個のマーカーが高い信頼度で注文されました (オッズ比> 1,000:1).
この地図は,推定ヒトゲノム長さの約90%をカバーしています.

結論:

開発されたリンクマップは,ヒトゲノムの密集的かつ秩序ある枠組みを提供します.
このリソースは,遺伝子マッピング,遺伝子関連研究,比較ゲノミクスを容易にします.
地図の広範なカバーは,ヒトゲノム研究における重要な進歩を表しています.

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