B細胞抗原受容体複合体:Ig-alphaとIg-betaが異なる細胞質エフェクターと関連している
M R Clark1, K S Campbell, A Kazlauskas
1Division of Basic Sciences, National Jewish Center for Immunology and Respiratory Medicine, Denver, CO 80206.
Science (New York, N.Y.)
|October 2, 1992
まとめ
B細胞抗原受容体複合体である.
科学分野:
- 免疫学 免疫学とは
- 分子生物学は分子生物学である.
- 細胞シグナル伝達 細胞信号伝達
背景:
- B細胞抗原受容体 (BCR) 複合体は,抗原認識と信号伝達を促進します.
- Ig-alphaとIg-betaサブユニットで構成されるBCRのトランスデューサー-トランスポーターサブ構造は,細胞質エフェクタ分子と相互作用しますが,相互作用の場所は不明でした.
- SrcファミリーキナーゼはBCR結合時に活性化されますが,受容体サブユニットの特定の結合部位は未特定でした.
研究 の 目的:
- Srcファミリーキナーゼを含む,B細胞抗原受容体複合体のIg-alphaおよびIg-betaサブユニットにおける,サイトプラズミックエフェクター分子の特定の結合部位を特定する.
- BCR媒介信号伝達経路におけるこれらの相互作用の役割を明らかにする.
主な方法:
- コイムノプレシピテーションは,受容体サブユニットとエフェクタ分子間のタンパク質対タンパク質の相互作用を特定するための測定法です.
- 効果分子結合のためのIg-alphaとIg-betaの細胞質尾領域の分析.
- サイト指向型変異および配列分析により,受容体サブユニット内の重要な結合モチーフを特定します.
主要な成果:
- 異なるエフェクター分子のセットは,Ig-alphaとIg-betaの細胞質尾と関連しています.
- Ig-α サイトプラズマドメインは,Lyn,Fyn,フォスファディチルノシトール-3 キナーゼ (PI-3 キナーゼ) と38kDaのフォスフォタンパク質を結合する.
- Ig-β 細胞質尾は,PI-3 キナーゼと40/42-kDaのフォスフォタンパク質に結合し,結合は保存された26アミノ酸モチーフによって媒介されます.
結論:
- Ig-alphaとIg-betaサブユニットは,独立したサイトプラズマ効果分子と独立して結合する.
- Ig-alphaとIg-betaの両方の保存された26アミノ酸モチーフは,エフェクタ分子への結合を媒介し,信号伝達のための保存されたメカニズムを示唆します.
- これらの発見は,B細胞抗原受容体の下流で異なる第2メッセンジャー経路の開始に不可欠な特定の相互作用部位を明らかにします.
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