ペプチド結合の抗体触媒による再配置
R A Gibbs1, S Taylor, S J Benkovic
1Department of Chemistry, Pennsylvania State University, University Park 16802.
Science (New York, N.Y.)
|October 30, 1992
まとめ
研究者らは,アスパラギニル-グリシル配列を変換する反応を触媒化する抗体を生成した. これらの抗体は,タンパク質とペプチドの機能を in vivo で無効化する新しい方法を提供します.
科学分野:
- バイオケミストリー バイオケミストリー
- 免疫学 免疫学とは
- 化学生物学 化学生物学とは
背景:
- タンパク質とペプチドは,アスパラジン残基の自発的なデアミデーションを経験します.
- この脱アミデーションは,イソアスパルチル結合の形成につながり,タンパク質の構造と機能を変化させます.
- タンパク質の機能を in vivo で制御する現在の方法は限られている.
研究 の 目的:
- アスパラギニルグリシル配列を効率的に処理できる新しい触媒抗体を開発する.
- これらの抗体がタンパク質とペプチドを in vivo で無効化する可能性を調査する.
主な方法:
- 双機能性サイクリック・フォスフィナート・トランジション・ステート・アナログを使用して抗体の生成.
- アスパラジニルグリシル基板の抗体触媒変換の特徴.
- サクシニミド中間物質の加工における抗体の活性度の評価.
主要な成果:
- 変換の両方のステップを効率的に触媒化する抗体を成功裏に生成した.
- サクシニミド中間体による反応の抗体媒介による触媒が実証された.
- アスパルチルグリシルおよびアイソアスパルチルグリシル製品の形成を特定しました.
結論:
- 触媒抗体は,アスパラギニル-グリシル配列を効果的に処理することができます.
- この抗体ベースのアプローチは,タンパク質とペプチドの in vivo 機能的無効化のための新しい戦略を提供し,従来のアミド分裂方法を補完します.
関連する概念動画
01:10Antibody Structure
Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
02:43Peptide Bonds
A peptide bond covalently attaches amino acids through a dehydration reaction. One amino acid's carboxyl group and another amino acid's amino group combine, releasing a water molecule. The resulting bond is the peptide bond. The products that such linkages form are peptides. As more amino acids join this growing chain, the resulting chain is a polypeptide. Each polypeptide has a free amino group at one end. This end has the N-terminal, or the amino-terminal, and the other end has a free...
01:24Protein Organization
Proteins are polymers of amino acid residues. They are versatile and responsible for different cellular functions, including DNA replication, molecular transport, catalysis, and structural support. Proteins have a hierarchical structure comprising at least three levels of organization: primary, secondary, and tertiary structure. Some large proteins have a quaternary structure where individual protein subunits are linked together.
The primary structure of a protein is its amino acid sequence.
The primary structure of a protein is its amino acid sequence.
01:25Protein Folding
Proteins are chains of amino acids linked together by peptide bonds. Upon synthesis, a protein folds into a three-dimensional conformation, critical to its biological function. Interactions between its constituent amino acids guide protein folding, and hence the protein structure is primarily dependent on its amino acid sequence.
Protein Structure Is Critical to Its Biological Function
Proteins perform a wide range of biological functions such as catalyzing chemical reactions, providing...
Protein Structure Is Critical to Its Biological Function
Proteins perform a wide range of biological functions such as catalyzing chemical reactions, providing...
01:25Antibody Structure and Classes
Antibodies, also known as immunoglobulins, are produced by B cells in response to foreign substances, such as bacteria and viruses. These proteins are critical for recognizing and neutralizing these substances, protecting the body from potential harm.
The basic structure of an antibody consists of four protein chains: two identical heavy chains and two identical light chains. These chains are held together by disulfide bonds and other non-covalent interactions, forming a Y-shaped structure.
The basic structure of an antibody consists of four protein chains: two identical heavy chains and two identical light chains. These chains are held together by disulfide bonds and other non-covalent interactions, forming a Y-shaped structure.
01:26Antibody Actions
Antibodies, or immunoglobulins, are critical players in the immune system's arsenal against invading pathogens. Produced by B cells and plasma cells, their primary role is to detect and bind to specific antigens, molecules found on the surface of pathogens like bacteria or viruses. Beyond antigen recognition, antibodies perform several vital functions that contribute to immune defense.
Neutralization
Antibodies can bind to pathogens, preventing them from infecting host cells. This process...
Neutralization
Antibodies can bind to pathogens, preventing them from infecting host cells. This process...