機能的なsiRNAsとmiRNAsは,鎖バイアスを示す
Anastasia Khvorova1, Angela Reynolds, Sumedha D Jayasena
1Amgen, Inc., One Amgen Center Drive, Thousand Oaks, CA 91320, USA.
Cell
|October 22, 2003
まとめ
小型の干渉RNA (siRNA) とマイクロRNA (miRNA) の機能は類似しています. siRNAの5'端の安定性は,その機能と長寿に不可欠であり,RNA干渉プロセスに影響を与えます.
科学分野:
- 分子生物学は分子生物学である.
- バイオケミストリー バイオケミストリー
- 遺伝学 遺伝学とは
背景:
- マイクロRNA (miRNA) と小干渉RNA (siRNA) は,遺伝子発現の重要な調節因子である.
- 両方ともDicerとRNA誘発静止複合体 (RISC) を利用してRNA干渉を行う.
- これらの分子の生体物理的性質を理解することは,それらの応用に不可欠です.
研究 の 目的:
- miRNA前駆体とsiRNA二重複体の内部安定性とその生物学的機能との関係を調査する.
- 機能的なsiRNA分子に関連する熱力学シグネチャを特定する.
主な方法:
- 公開されたmiRNA前駆体配列の統計分析.
- 機能的および非機能的siRNA二重複体の内部安定性の分析.
- 植物細胞から採取したsiRNA分子の熱力学分析.
主要な成果:
- miRNA前駆体は,特に5'-anti-sense (AS) 端末ベースペアで,柔軟性が向上しています.
- 機能的なsiRNAデュプレックスは,非機能的なものと比較して,5'-AS端でより低い内部安定性を示しています.
- 植物細胞のsiRNA分子は,機能に関連した特徴的な熱力学シグネチャーを示します.
結論:
- siRNAの熱力学的特性,特に5'-AS端での内部安定性は,その機能と持続性にとって重要である.
- これらの性質は,二重展開解きやRISC介在の鎖保持などのRNA干渉の重要なステップに影響を与える可能性があります.
関連する概念動画
01:22MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
01:23RNA Interference
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
01:22MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
02:30siRNA - Small Interfering RNAs
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
03:21RNA-seq
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
01:22MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...