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DNA Isolation

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DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

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Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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関連する実験動画

Updated: Jul 18, 2026

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis

Published on: June 19, 2018

DNA結合タンパク質を分析ツールとして使用する.

Maxim Berezovski¹, Sergey N Krylov

¹Department of Chemistry, York University, Toronto, Ontario M3J 1P3, Canada.

Journal of the American Chemical Society

|October 30, 2003

まとめ

DNA結合タンパク質は,DNA,RNA,およびタンパク質を分析するための汎用的なツールを提供します. 毛細血管電泳で単一鎖DNA結合タンパク質 (SSB) を利用することで,これらの分子の効率的なゲルなしの分離と定量化が可能になります.

科学分野:

バイオケミストリーバイオケミストリー
分子生物学は分子生物学である.
アナリティカル・ケミストリー (Analytical Chemistry) とは

背景:

伝統的な親和性分析では,電離分離のためにシート行列をシートする必要がしばしばあります.
ゲルフリーシステムでは,プローブとプローブターゲット複合体の区別が難しいことがあります.

研究の目的:

DNA結合タンパク質を使用してDNA,RNA,およびタンパク質を分析するための多用途かつ効率的な方法を開発する.
毛細血管電泳における親和感探査機とその標的のゲルフリー分離と定量化を実証する.

主な方法:

単一鎖DNAである特定の親近性プローブ (ハイブリダイゼーションとアプタマープローブ) を利用しました.
毛細血管電泳 (CE) の単一鎖DNA結合タンパク質 (SSB) が使用されている.
モビリティの差異を誘導するために,プローブとプローブターゲット複合体へのSSBの差分結合を活用しました.

主要な成果:

CEでSSBを使用して,DNA,RNA,およびタンパク質の標的の高効率のゲルフリー分離を達成しました.
SSBの濃度に依存して,探査機とその複合体の電泳的運動性を変化させることが示された.
シービングマトリックスを必要とせずにDNA,RNA,タンパク質の親和性分析を可能にしました.

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Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

関連する実験動画

Last Updated: Jul 18, 2026

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis

Published on: June 19, 2018

DNAzyme 10-23 - Based Nanomachines for Nucleic Acid Recognition

DNAzyme 10-23 - Based Nanomachines for Nucleic Acid Recognition

Published on: February 9, 2024

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

結論:

SSBで示されたDNA結合タンパク質は,多用途の分子分析のための強力なツールキットを提供します.
このゲルなしのCE方法は,DNA,RNA,タンパク質の正確な定量化を促進します.
潜在的な応用には,ゲノムDNAの識別と,mRNAとタンパク質レベルで遺伝子発現のモニタリングが含まれます.